Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli

Robert Malinowski, Stephen C. Fry, Sabina Zuzga, Anita Wiśniewska, Michał Godlewski, Andrzej Noyszewski, Anna Barczak‑Brzyżek, Stefan Malepszy, Marcin Filipecki

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Abstract

The expression of two genes encoding xyloglucan endotransglucosylase/hydrolases (XTHs), Cs-XTH1 and Cs-XTH3, was upregulated during the onset of cucumber somatic embryogenesis. As a means of characterising the developmental regulation of these genes, the activity of the respective upstream regulatory regions was investigated in seedlings and somatic embryos of Arabidopsis thaliana and Cucumis sativus. GUS assays revealed that both genes are under developmental control. In addition, elevated promoter activity was found in the tension-bearing regions of the plant and in response to touch and wounding, which is consistent with the existence of numerous stress-related cis elements in the 5′-regulatory regions. In vivo xyloglucan endotransglucosylase (XET) action assays were performed to gain an overview on the role of XTHs during somatic embryogenesis. The highest XET action was observed in the external cell layers of somatic embryos in the cotyledonary region and in the presumptive region of peg formation. Based on the results, we propose a dual mechanism (one developmental and the second adaptive) for the regulation of Cs-XTH1 and Cs-XTH3 activity wherein the developmental pattern can be modified by mechanical stimuli.
Original languageEnglish
Pages (from-to)130
Number of pages11
JournalActa Physiologiae Plantarum
Volume40
DOIs
Publication statusPublished - 29 Jun 2018

Keywords

  • Xyloglucan endotransglucosylase/hydrolase (XTH)
  • Somatic embryogenesis
  • Cucumber
  • Cucumis sativus
  • Developmental and adaptive gene regulation
  • Xyloglucan–oligosaccharide–sulphorhodamine conjugate (XGO-SR)

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