DGCR8 acts as an adaptor for the exosome complex to degrade double-stranded structured RNAs

Sara Macias Ribela; Ross Cordiner; Phillipe Gautier; Mireya Plass; Javier F. Cáceres

doi:10.1016/j.molcel.2015.11.011

DGCR8 acts as an adaptor for the exosome complex to degrade double-stranded structured RNAs

Sara Macias Ribela, Ross Cordiner, Phillipe Gautier, Mireya Plass, Javier F. Cáceres

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

The Microprocessor complex (DGCR8/Drosha) is required for microRNA (miRNA) biogenesis but also binds and regulates the stability of several types of cellular RNAs. Of particular interest, DGCR8 controls the stability of mature small nucleolar RNA (snoRNA) transcripts independently of Drosha, suggesting the existence of alternative DGCR8 complex(es) with other nucleases to process a variety of cellular RNAs. Here, we found that DGCR8 copurifies with subunits of the nuclear exosome, preferentially associating with its hRRP6-containing nucleolar form. Importantly, we demonstrate that DGCR8 is essential for the recruitment of the exosome to snoRNAs and to human telomerase RNA. In addition, we show that the DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC). Altogether, these data suggest that DGCR8 acts as an adaptor to recruit the exosome complex to structured RNAs and induce their degradation.

Original language	English
Pages (from-to)	873-885
Number of pages	13
Journal	Molecular Cell
Volume	60
Issue number	6
Early online date	10 Dec 2015
DOIs	https://doi.org/10.1016/j.molcel.2015.11.011
Publication status	Published - 17 Dec 2015

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CROATIA PROGRAMME
Wright, A., Adams, I., Aligianis, I., Baldock, R., Bickmore, W., Caceres, J., Dorin, J., Dunlop, M., FitzPatrick, D., Haley, C., Hastie, N., Hill, B., Jackson, I., Jackson, A., Kudla, G., Meehan, R., O'Connell, M., Overton, I., Patton, E., Taylor, M., Tenesa, A. & Van Heyningen, V.
MRC
1/04/12 → 31/07/13
Project: Research
RNA BINDING PROTEINS IN HEALTH AND DISEASE
Caceres, J.
UK-based charities
1/10/11 → 31/03/17
Project: Research

Javier Caceres
- Javier.Caceresigmm.ed.acuk
- javier.caceresed.acuk
- Deanery of Molecular, Genetic and Population Health Sciences - Personal Chair of RNA and Gene Expression
- MRC Human Genetics Unit
Person: Academic: Research Active
Sara Macias Ribela
- sara.maciasribelaed.acuk
- School of Biological Sciences - Wellcome Senior Research Fellow
Person: Academic: Research Active

Cite this

@article{a68cec3979aa488084ea215a8e529b31,

title = "DGCR8 acts as an adaptor for the exosome complex to degrade double-stranded structured RNAs",

abstract = "The Microprocessor complex (DGCR8/Drosha) is required for microRNA (miRNA) biogenesis but also binds and regulates the stability of several types of cellular RNAs. Of particular interest, DGCR8 controls the stability of mature small nucleolar RNA (snoRNA) transcripts independently of Drosha, suggesting the existence of alternative DGCR8 complex(es) with other nucleases to process a variety of cellular RNAs. Here, we found that DGCR8 copurifies with subunits of the nuclear exosome, preferentially associating with its hRRP6-containing nucleolar form. Importantly, we demonstrate that DGCR8 is essential for the recruitment of the exosome to snoRNAs and to human telomerase RNA. In addition, we show that the DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC). Altogether, these data suggest that DGCR8 acts as an adaptor to recruit the exosome complex to structured RNAs and induce their degradation.",

author = "{Macias Ribela}, Sara and Ross Cordiner and Phillipe Gautier and Mireya Plass and C{\'a}ceres, {Javier F.}",

year = "2015",

month = dec,

day = "17",

doi = "10.1016/j.molcel.2015.11.011",

language = "English",

volume = "60",

pages = "873--885",

journal = "Molecular Cell",

issn = "1097-2765",

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number = "6",

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T1 - DGCR8 acts as an adaptor for the exosome complex to degrade double-stranded structured RNAs

AU - Macias Ribela, Sara

AU - Cordiner, Ross

AU - Gautier, Phillipe

AU - Plass, Mireya

AU - Cáceres, Javier F.

PY - 2015/12/17

Y1 - 2015/12/17

N2 - The Microprocessor complex (DGCR8/Drosha) is required for microRNA (miRNA) biogenesis but also binds and regulates the stability of several types of cellular RNAs. Of particular interest, DGCR8 controls the stability of mature small nucleolar RNA (snoRNA) transcripts independently of Drosha, suggesting the existence of alternative DGCR8 complex(es) with other nucleases to process a variety of cellular RNAs. Here, we found that DGCR8 copurifies with subunits of the nuclear exosome, preferentially associating with its hRRP6-containing nucleolar form. Importantly, we demonstrate that DGCR8 is essential for the recruitment of the exosome to snoRNAs and to human telomerase RNA. In addition, we show that the DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC). Altogether, these data suggest that DGCR8 acts as an adaptor to recruit the exosome complex to structured RNAs and induce their degradation.

AB - The Microprocessor complex (DGCR8/Drosha) is required for microRNA (miRNA) biogenesis but also binds and regulates the stability of several types of cellular RNAs. Of particular interest, DGCR8 controls the stability of mature small nucleolar RNA (snoRNA) transcripts independently of Drosha, suggesting the existence of alternative DGCR8 complex(es) with other nucleases to process a variety of cellular RNAs. Here, we found that DGCR8 copurifies with subunits of the nuclear exosome, preferentially associating with its hRRP6-containing nucleolar form. Importantly, we demonstrate that DGCR8 is essential for the recruitment of the exosome to snoRNAs and to human telomerase RNA. In addition, we show that the DGCR8/exosome complex controls the stability of the human telomerase RNA component (hTR/TERC). Altogether, these data suggest that DGCR8 acts as an adaptor to recruit the exosome complex to structured RNAs and induce their degradation.

U2 - 10.1016/j.molcel.2015.11.011

DO - 10.1016/j.molcel.2015.11.011

M3 - Article

SN - 1097-2765

VL - 60

SP - 873

EP - 885

JO - Molecular Cell

JF - Molecular Cell

IS - 6

ER -

DGCR8 acts as an adaptor for the exosome complex to degrade double-stranded structured RNAs

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Projects

CROATIA PROGRAMME

RNA BINDING PROTEINS IN HEALTH AND DISEASE

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Javier Caceres

Sara Macias Ribela

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