Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives

Srinivas Velugotla, Steve Pells, Heidi K Mjoseng, Cairnan R E Duffy, Stewart Smith, Paul de Sousa, Ronald Pethig

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Assessment of the dielectrophoresis (DEP) cross-over frequency (fxo), cell diameter, and derivative membrane capacitance (Cm) values for a group of undifferentiated human embryonic stem cell (hESC) lines (H1, H9, RCM1, RH1), and for a transgenic subclone of H1 (T8) revealed that hESC lines could not be discriminated on their mean fxo and Cm values, the latter of which ranged from 14 to 20 mF/m2. Differentiation of H1 and H9 to a mesenchymal stem cell-like phenotype resulted in similar significant increases in mean Cm values to 41–49 mF/m2 in both lines (p < 0.0001). BMP4-induced differentiation of RCM1 to a trophoblast cell-like phenotype also resulted in a distinct and significant increase in mean Cm value to 28 mF/m2 (p < 0.0001). The progressive transition to a higher membrane capacitance was also evident after each passage of cell culture as H9 cells transitioned to a mesenchymal stem cell-like state induced by growth on a substrate of hyaluronan. These findings confirm the existence of distinctive parameters between undifferentiated and differentiating cells on which future application of dielectrophoresis in the context of hESC manufacturing can be based.

Original languageEnglish
Article number044113
Number of pages12
JournalBiomicrofluidics
Volume6
Issue number4
DOIs
Publication statusPublished - Dec 2012

Keywords / Materials (for Non-textual outputs)

  • bioelectric phenomena
  • biomembranes
  • capacitance
  • cellular biophysics
  • electrophoresis

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