Projects per year
Abstract / Description of output
Dielectrophoresis (DEP) is a label-free technique for the characterization and manipulation of biological particles - such as cells, bacteria and viruses. Many studies have focused on the DEP cross-over frequency fxo1, where cells in a non-uniform electric field undergo a transition from negative to positive DEP. Determination of fxo1 provides a value for the membrane capacitance from the cell diameter, the means to monitor changes in cell morphology and viability, and the information required when devising DEP cell separation protocols. In this paper we describe the first systematic measurements of the second DEP cross-over frequency fxo2 that occurs at much higher frequencies. Theory indicates that fxo2 is sensitive to the internal dielectric properties of a cell, and our experiments on murine myeloma cells reveal that these properties exhibit temporal changes that are sensitive to both the osmolality and temperature of the cell suspending medium.
Original language | English |
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Pages (from-to) | 64-71 |
Number of pages | 8 |
Journal | Journal of Electrical Bioimpedance |
Volume | 2 |
Issue number | 2011 |
DOIs | |
Publication status | Published - 7 Jan 2011 |
Keywords / Materials (for Non-textual outputs)
- Biodielectrics
- Cytoplasm Conductivity
- Dielectro-phoresis
- Myeloma Cells
- Nucleus conductivity
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Dive into the research topics of 'Dielectrophoretic Characterisation of Mammalian Cells above 100 MHz'. Together they form a unique fingerprint.Projects
- 1 Finished
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RCUK Fellowship
Smith, S., Anderton, S., Bagnaninchi, P., Blythe, R., Calvert, J., Downes, A., Frame, M., Hay, D., Popovic, N. & Rambaut, A.
1/10/06 → 30/06/13
Project: Research