Differences in the induction of CD8+ T cell responses by subpopulations of dendritic cells from afferent lymph are related to IL-1 alpha secretion

Jayne Hope, Paul Sopp, R A Collins, Chris J Howard

Research output: Contribution to journalArticlepeer-review

Abstract

The major subset of dendritic cells (DC) from bovine afferent lymph expresses the SIRP alpha MyD-1 antigen, but not CD11a or the antigen recognized by mAb CC81, and potently stimulates CD4+ and CD8+ T lymphocyte proliferation. The minor subpopulation, that is CD11a+ CC81+ MyD-1-, effectively stimulates CD4+ but not CD8+ T lymphocyte proliferation. CD11a+ CC81+ MyD-1- DC did not induce anergy or death or secrete an inhibitory factor. However, supernatant from cultures of CD8+ T cells with CD11a- CC81- MyD-1+ DC significantly enhanced proliferation of CD8+ T cells in response to CD11a+ CC81+ MyD-1- DC, an effect that was blocked by interleukin (IL)-1alpha, but not IL-1beta, specific mAb. The proliferation of CD8+ T cells with CD11a+ CC81+ MyD-1- DC was also enhanced by adding IL-1alpha. IL-1beta slightly enhanced proliferation, whereas IL-2, IL-6, IL-12, and IL-15 had no effect. We conclude that the failure to stimulate CD8+ T cell proliferation results from the lack of IL-1alpha synthesis by this population, which may have important consequences in vivo.
Original languageEnglish
Pages (from-to)271-9
Number of pages9
JournalJournal of Leukocyte Biology
Volume69
Issue number2
Publication statusPublished - Feb 2001

Keywords

  • Adjuvants, Immunologic/genetics
  • Adjuvants, Immunologic/pharmacology
  • Animals
  • Antibodies, Monoclonal/analysis
  • Antigens, CD11/biosynthesis
  • Antigens, CD11/immunology
  • CD8-Positive T-Lymphocytes/cytology
  • CD8-Positive T-Lymphocytes/immunology
  • Cattle
  • Cell Separation
  • Cell Survival/immunology
  • Cell-Free System/immunology
  • Cells, Cultured
  • Clonal Anergy
  • Cytotoxicity, Immunologic
  • Dendritic Cells/immunology
  • Dendritic Cells/metabolism
  • Down-Regulation/immunology
  • Humans
  • Immunophenotyping
  • Interleukin-1
  • Interleukin-12
  • Interleukin-2
  • Interleukin-5
  • Interleukin-6
  • Isoantigens/immunology
  • Lymph/cytology
  • Lymph/immunology
  • Lymphocyte Activation
  • Recombinant Proteins/immunology
  • Recombinant Proteins/pharmacology

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