Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Hassan Rashidi; Stephanie Strohbuecker; Leigh Jackson; Spandan Kalra; Alexander J. Blake; Louise France; Cristina Tufarelli; Virginie Sottile

doi:10.1159/000329861

Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Hassan Rashidi, Stephanie Strohbuecker, Leigh Jackson, Spandan Kalra, Alexander J. Blake, Louise France, Cristina Tufarelli, Virginie Sottile^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling. Copyright (C) 2011 S. Karger AG, Basel

Original language	English
Pages (from-to)	484-494
Number of pages	11
Journal	Cells tissues organs
Volume	195
Issue number	6
DOIs	https://doi.org/10.1159/000329861
Publication status	Published - 2012

Keywords / Materials (for Non-textual outputs)

Osteogenic treatment
Mineral deposition
Mesenchymal stem cell
Cancer cell line
MESENCHYMAL STEM-CELLS
EMBRYONAL CARCINOMA-CELLS
BONE-DERIVED CELLS
IN-VITRO
OSTEOBLAST DIFFERENTIATION
BETA-GLYCEROPHOSPHATE
TISSUE-CULTURE
BREAST-CANCER
STROMAL CELLS
MARROW

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@article{cc5825299d07407b8cf9ad860053e792,

title = "Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin",

abstract = "Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling. Copyright (C) 2011 S. Karger AG, Basel",

keywords = "Osteogenic treatment, Mineral deposition, Mesenchymal stem cell, Cancer cell line, MESENCHYMAL STEM-CELLS, EMBRYONAL CARCINOMA-CELLS, BONE-DERIVED CELLS, IN-VITRO, OSTEOBLAST DIFFERENTIATION, BETA-GLYCEROPHOSPHATE, TISSUE-CULTURE, BREAST-CANCER, STROMAL CELLS, MARROW",

author = "Hassan Rashidi and Stephanie Strohbuecker and Leigh Jackson and Spandan Kalra and Blake, {Alexander J.} and Louise France and Cristina Tufarelli and Virginie Sottile",

year = "2012",

doi = "10.1159/000329861",

language = "English",

volume = "195",

pages = "484--494",

journal = "Cells tissues organs",

issn = "1422-6405",

publisher = "S. Karger AG",

number = "6",

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TY - JOUR

T1 - Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

AU - Rashidi, Hassan

AU - Strohbuecker, Stephanie

AU - Jackson, Leigh

AU - Kalra, Spandan

AU - Blake, Alexander J.

AU - France, Louise

AU - Tufarelli, Cristina

AU - Sottile, Virginie

PY - 2012

Y1 - 2012

N2 - Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling. Copyright (C) 2011 S. Karger AG, Basel

AB - Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling. Copyright (C) 2011 S. Karger AG, Basel

KW - Osteogenic treatment

KW - Mineral deposition

KW - Mesenchymal stem cell

KW - Cancer cell line

KW - MESENCHYMAL STEM-CELLS

KW - EMBRYONAL CARCINOMA-CELLS

KW - BONE-DERIVED CELLS

KW - IN-VITRO

KW - OSTEOBLAST DIFFERENTIATION

KW - BETA-GLYCEROPHOSPHATE

KW - TISSUE-CULTURE

KW - BREAST-CANCER

KW - STROMAL CELLS

KW - MARROW

U2 - 10.1159/000329861

DO - 10.1159/000329861

M3 - Article

SN - 1422-6405

VL - 195

SP - 484

EP - 494

JO - Cells tissues organs

JF - Cells tissues organs

IS - 6

ER -

Differences in the Pattern and Regulation of Mineral Deposition in Human Cell Lines of Osteogenic and Non-Osteogenic Origin

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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