Different vaccine strategies used to protect against Theileria annulata

NR Boulter*, CGD Brown, E Kirvar, E Glass, J Campbell, S Morzaria, V. Nene, A Musoke, C D'Oliveira, MJ Gubbels, F Jongejan, FR Hall

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

SPAG-1, a sporozoite surface antigen of T. annulata, has previously been shown to elicit partial protection when used, as an hepatitis B core antigen fusion, to immunize cattle. The objective of this study was to try and improve the protective capacity of this antigen by enlisting different vaccine strategics. Cattle were immunized with SPAG-1, as a fusion protein with a His(6) tag, either incorporated into ISCOMs, with or without the merozoite antigens TAMS 1-1 and 1-2, or with RWL as adjuvant three times at monthly intervals. Another group of cattle ware immunized with p67, the T. parva sporozoite antigen, in RWL to assess whether any cross-protection could be induced. The animals were then challenged with an estimated LD50 of T. annulata sporozoites, and their ability to resist the infection was investigated, Serum responses and T-cell proliferative responses were analyzed throughout the trial, Post-challenge analyses included lymph node biopsies and blood smears to check for the presence of parasites, routine hematological parameters, and observation for clinical manifestations of the disease. The results of this trial will be discussed.

Original languageEnglish
Title of host publicationTROPICAL VETERINARY MEDICINE
EditorsF Jongejan, W Goff, E Camus
Place of PublicationNEW YORK
PublisherNew York Academy of Sciences
Pages234-246
Number of pages13
ISBN (Print)1-57331-141-3
Publication statusPublished - 1998
Event4th Biennial Meeting of the Society-for-Tropical-Veterinary-Medicine - MONTPELLIER, France
Duration: 5 May 19979 May 1997

Publication series

NameANNALS OF THE NEW YORK ACADEMY OF SCIENCES
PublisherNEW YORK ACAD SCIENCES
Volume849
ISSN (Print)0077-8923

Conference

Conference4th Biennial Meeting of the Society-for-Tropical-Veterinary-Medicine
CountryFrance
CityMONTPELLIER
Period5/05/979/05/97

Keywords

  • SPOROZOITE SURFACE-ANTIGEN
  • NEUTRALIZING DETERMINANTS
  • SPAG-1
  • PARVA

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