Differential Dynamic Microscopy of Bacterial Motility

L. G. Wilson; V. A. Martinez; J. Schwarz-Linek; J. Tailleur; G. Bryant; P. N. Pusey; Wilson Poon

doi:10.1103/PhysRevLett.106.018101

Differential Dynamic Microscopy of Bacterial Motility

L. G. Wilson, V. A. Martinez, J. Schwarz-Linek, J. Tailleur, G. Bryant, P. N. Pusey, Wilson Poon

School of Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

We demonstrate a method for the fast, high-throughput characterization of the dynamics of active particles. Specifically, we measure the swimming speed distribution and motile cell fraction in Escherichia coli suspensions. By averaging over similar to 10(4) cells, our method is highly accurate compared to conventional tracking, yielding a routine tool for motility characterization. We find that the diffusivity of nonmotile cells is enhanced in proportion to the concentration of motile cells.

Original language	English
Article number	018101
Pages (from-to)	-
Number of pages	4
Journal	Physical Review Letters
Volume	106
Issue number	1
DOIs	https://doi.org/10.1103/PhysRevLett.106.018101
Publication status	Published - 5 Jan 2011

Keywords / Materials (for Non-textual outputs)

PHOTON-CORRELATION SPECTROSCOPY
HELICOBACTER-PYLORI
ESCHERICHIA-COLI
TRACKING
DIFFUSION

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10.1103/PhysRevLett.106.018101

Cite this

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title = "Differential Dynamic Microscopy of Bacterial Motility",

abstract = "We demonstrate a method for the fast, high-throughput characterization of the dynamics of active particles. Specifically, we measure the swimming speed distribution and motile cell fraction in Escherichia coli suspensions. By averaging over similar to 10(4) cells, our method is highly accurate compared to conventional tracking, yielding a routine tool for motility characterization. We find that the diffusivity of nonmotile cells is enhanced in proportion to the concentration of motile cells.",

keywords = "PHOTON-CORRELATION SPECTROSCOPY, HELICOBACTER-PYLORI, ESCHERICHIA-COLI, TRACKING, DIFFUSION",

author = "Wilson, {L. G.} and Martinez, {V. A.} and J. Schwarz-Linek and J. Tailleur and G. Bryant and Pusey, {P. N.} and Wilson Poon",

year = "2011",

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doi = "10.1103/PhysRevLett.106.018101",

language = "English",

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TY - JOUR

T1 - Differential Dynamic Microscopy of Bacterial Motility

AU - Wilson, L. G.

AU - Martinez, V. A.

AU - Schwarz-Linek, J.

AU - Tailleur, J.

AU - Bryant, G.

AU - Pusey, P. N.

AU - Poon, Wilson

PY - 2011/1/5

Y1 - 2011/1/5

N2 - We demonstrate a method for the fast, high-throughput characterization of the dynamics of active particles. Specifically, we measure the swimming speed distribution and motile cell fraction in Escherichia coli suspensions. By averaging over similar to 10(4) cells, our method is highly accurate compared to conventional tracking, yielding a routine tool for motility characterization. We find that the diffusivity of nonmotile cells is enhanced in proportion to the concentration of motile cells.

AB - We demonstrate a method for the fast, high-throughput characterization of the dynamics of active particles. Specifically, we measure the swimming speed distribution and motile cell fraction in Escherichia coli suspensions. By averaging over similar to 10(4) cells, our method is highly accurate compared to conventional tracking, yielding a routine tool for motility characterization. We find that the diffusivity of nonmotile cells is enhanced in proportion to the concentration of motile cells.

KW - PHOTON-CORRELATION SPECTROSCOPY

KW - HELICOBACTER-PYLORI

KW - ESCHERICHIA-COLI

KW - TRACKING

KW - DIFFUSION

UR - http://www.scopus.com/inward/record.url?scp=78650985996&partnerID=8YFLogxK

U2 - 10.1103/PhysRevLett.106.018101

DO - 10.1103/PhysRevLett.106.018101

M3 - Article

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VL - 106

SP - -

JO - Physical Review Letters

JF - Physical Review Letters

IS - 1

M1 - 018101

ER -

Differential Dynamic Microscopy of Bacterial Motility

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Edinbugrh Soft Matter and Statistical Physics Programme Grant Renewal

Cite this

Differential Dynamic Microscopy of Bacterial Motility

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

Edinbugrh Soft Matter and Statistical Physics Programme Grant Renewal

Cite this