Differential Enzymatic 16O/18O Labeling for the Detection of Cross-Linked Nucleic Acid-Protein Heteroconjugates

Fiona J Flett, Timo Sachsenberg, Oliver Kohlbacher, C Logan Mackay, Heidrun Interthal

Research output: Contribution to journalArticlepeer-review

Abstract

Cross-linking of nucleic acids to proteins in combination with mass spectrometry permits the precise identification of interacting residues between nucleic acid-protein complexes. However, the mass spectrometric identification and characterization of cross-linked nucleic acid-protein heteroconjugates within a complex sample is challenging. Here we establish a novel enzymatic differential16O/18O-labeling approach, which uniquely labels heteroconjugates. We have developed an automated data analysis workflow based on OpenMS for the identification of differentially isotopically labeled heteroconjugates against a complex background. We validated our method using synthetic model DNA oligonucleotide-peptide heteroconjugates, which were subjected to the labeling reaction and analyzed by high-resolution FTICR mass spectrometry.

Original languageEnglish
Pages (from-to)11208-11213
Number of pages6
JournalAnalytical Chemistry
Volume89
Issue number21
Early online date8 Sep 2017
DOIs
Publication statusPublished - 7 Nov 2017

Fingerprint

Dive into the research topics of 'Differential Enzymatic 16O/18O Labeling for the Detection of Cross-Linked Nucleic Acid-Protein Heteroconjugates'. Together they form a unique fingerprint.

Cite this