Differential expression of cyclin-dependent kinase inhibitors and apoptosis-related proteins in endocervical lesions

Alaa A El-Ghobashy, Abeer M Shaaban, Jenny Innes, Wendy Prime, C Simon Herrington

Research output: Contribution to journalArticlepeer-review

Abstract

The development of neoplasia is associated with abnormalities of cell cycle control and apoptosis. In this study, a panel of cyclin-dependent kinase inhibitors (CDKIs) and apoptosis-related proteins (p16, p21, p53, Bcl2 and hsp27) was analysed by immunohistochemistry in 91 glandular cervical lesions. A significant increase in p21 and p53 expression occurred from normal cervix (n=11) through endometriosis/tubo-endometrioid metaplasia (TEM) (n=19) and cervical glandular intraepithelial neoplasia (CGIN)/adenocarcinoma in situ (AIS) (n=33) to invasive adenocarcinoma (n=28). p16 showed diffuse strong expression in CGIN/AIS and invasive adenocarcinoma compared with focal expression in some TEM/endometriosis lesions and no expression in normal cervix. Bcl2 was highly expressed in TEM/endometriosis compared with CGIN/AIS and adenocarcinoma. p16 immunostaining discriminated accurately between neoplastic and non-neoplastic cervical lesions, provided that diffuse strong positivity was present. Similarly, diffuse expression of Bcl2 distinguished endometriosis/TEM from CGIN/AIS. These data demonstrate that analysis of CDKIs and apoptosis-related proteins provides useful information in the diagnostic assessment of glandular lesions of the cervix.

Original languageEnglish
Pages (from-to)2011-8
Number of pages8
JournalEuropean journal of cancer (Oxford, England : 1990)
Volume43
Issue number13
DOIs
Publication statusPublished - Sep 2007

Keywords

  • Adenocarcinoma
  • Adult
  • Aged
  • Aged, 80 and over
  • Apoptosis
  • Cervical Intraepithelial Neoplasia
  • Cervix Uteri
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Tumor Markers, Biological
  • Uterine Cervical Neoplasms

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