Differential expression of sphingosine-1-phosphate receptors 1-5 in the developing nervous system

H Meng, V M Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Sphingosine-1-phosphate (S1P) binds to G protein-coupled receptors and can regulate a wide range of cellular functions. In a previous study, we isolated two key enzymes in the S1P pathway that were expressed in migrating neural crest cells. To determine if S1P receptors are present in neural crest cells or peripheral nervous system, we examine the expression patterns of S1P receptors (S1pr1-5) in mouse, and s1pr1 and s1pr3 in chick embryos. Here, we present a comprehensive expression analysis of these receptors using in situ hybridizations, which provide spatiotemporal information. We showed that S1pr2 was expressed in migrating cranial neural crest cells and enteric neurons. S1pr1 was prominently expressed in the neuroepithelium whereas S1pr4 and S1pr5 were in neurons at later stages. On the contrary, S1pr3 was predominantly detected in non-neuronal cells within and surrounding neural structures. We also described novel expression sites for S1P receptors in the developing nervous system.
Original languageEnglish
Pages (from-to)487-500
Number of pages14
JournalDevelopmental Dynamics
Volume238
Issue number2
DOIs
Publication statusPublished - Feb 2009

Keywords

  • Animals
  • Chick Embryo
  • Mice
  • Nervous System
  • Neurons
  • Receptors, G-Protein-Coupled
  • Receptors, Lysosphingolipid

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