TY - JOUR
T1 - Differential gene expression pattern of importin β3 and NS5 in C6/36 cells acutely and persistently infected with dengue virus 2
AU - Ávila-Ramírez, María Leticia
AU - Reyes-Reyes, Ana Laura
AU - Avila-Bonilla, Rodolfo Gamaliel
AU - Salas-Benito, Mariana
AU - Cerecedo, Doris
AU - Ramírez-Moreno, María Esther
AU - Villagrán-Herrera, María Elena
AU - Mercado-Curiel, Ricardo Francisco
AU - Salas-Benito, Juan Santiago
N1 - Funding Information:
This research was funded by SECRETARÍA DE INVESTIGACIÓN Y POSGRADO from INSTITUTO POLITÉCNICO NACIONAL, grant number SIP-20170965.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/1/27
Y1 - 2023/1/27
N2 - The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in Aedes albopictus cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate.
AB - The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in Aedes albopictus cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate.
KW - dengue virus
KW - viral persistence
KW - importin
KW - nuclear transport
U2 - 10.3390/pathogens12020191
DO - 10.3390/pathogens12020191
M3 - Article
VL - 12
JO - Pathogens
JF - Pathogens
SN - 2076-0817
IS - 2
M1 - 191
ER -