Differential global gene expression in cystic fibrosis nasal and bronchial epithelium

Varrie Ogilvie; Margaret Passmore; Laura Hyndman; Lisa Jones; Barbara Stevenson; Abigail Wilson; Heather Davidson; Robert R Kitchen; Robert D Gray; Pallav Shah; Eric W Alton; Jane C Davies; David J Porteous; A Christopher Boyd

doi:10.1016/j.ygeno.2011.06.008

Differential global gene expression in cystic fibrosis nasal and bronchial epithelium

Varrie Ogilvie, Margaret Passmore, Laura Hyndman, Lisa Jones, Barbara Stevenson, Abigail Wilson, Heather Davidson, Robert R Kitchen, Robert D Gray, Pallav Shah, Eric W Alton, Jane C Davies, David J Porteous, A Christopher Boyd

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Respiratory epithelium is the target of therapies, such as gene therapy, for cystic fibrosis (CF) lung disease. To determine the usefulness of the nasal epithelium as a pre-screen for lung-directed therapies, we profiled gene expression in CF and non-CF nasal and bronchial epithelium samples using Illumina HumanRef-8 Expression BeadChips. 863 genes were differentially expressed between CF and non-CF bronchial epithelium but only 15 were differentially expressed between CF and non-CF nasal epithelium (≥1.5-fold, P≤0.05). The most enriched pathway in CF bronchial epithelium was inflammatory response, whereas in CF nasal epithelium it was amino acid metabolism. We also compared nasal and bronchial epithelium in each group and identified differential expression of cellular movement genes in CF patients and cellular growth genes in non-CF subjects. We conclude that CF and non-CF nasal and bronchial epithelium are transcriptionally distinct and CF nasal epithelium is not a good surrogate for the lung respiratory epithelium.

Original language	English
Pages (from-to)	327-336
Number of pages	10
Journal	Genomics
Volume	98
Issue number	5
DOIs	https://doi.org/10.1016/j.ygeno.2011.06.008
Publication status	Published - 2011

Keywords / Materials (for Non-textual outputs)

Adolescent
Adult
Bronchi
Case-Control Studies
Child
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
Female
Gene Expression Regulation
Humans
Immunohistochemistry
Inflammation
Keratins
Male
Nasal Mucosa
Oligonucleotide Array Sequence Analysis
Young Adult

Access to Document

10.1016/j.ygeno.2011.06.008

Cite this

@article{32248ebeb29945e7b8d53d7c8a10b410,

title = "Differential global gene expression in cystic fibrosis nasal and bronchial epithelium",

abstract = "Respiratory epithelium is the target of therapies, such as gene therapy, for cystic fibrosis (CF) lung disease. To determine the usefulness of the nasal epithelium as a pre-screen for lung-directed therapies, we profiled gene expression in CF and non-CF nasal and bronchial epithelium samples using Illumina HumanRef-8 Expression BeadChips. 863 genes were differentially expressed between CF and non-CF bronchial epithelium but only 15 were differentially expressed between CF and non-CF nasal epithelium (≥1.5-fold, P≤0.05). The most enriched pathway in CF bronchial epithelium was inflammatory response, whereas in CF nasal epithelium it was amino acid metabolism. We also compared nasal and bronchial epithelium in each group and identified differential expression of cellular movement genes in CF patients and cellular growth genes in non-CF subjects. We conclude that CF and non-CF nasal and bronchial epithelium are transcriptionally distinct and CF nasal epithelium is not a good surrogate for the lung respiratory epithelium.",

keywords = "Adolescent, Adult, Bronchi, Case-Control Studies, Child, Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, Female, Gene Expression Regulation, Humans, Immunohistochemistry, Inflammation, Keratins, Male, Nasal Mucosa, Oligonucleotide Array Sequence Analysis, Young Adult",

author = "Varrie Ogilvie and Margaret Passmore and Laura Hyndman and Lisa Jones and Barbara Stevenson and Abigail Wilson and Heather Davidson and Kitchen, {Robert R} and Gray, {Robert D} and Pallav Shah and Alton, {Eric W} and Davies, {Jane C} and Porteous, {David J} and Boyd, {A Christopher}",

year = "2011",

doi = "10.1016/j.ygeno.2011.06.008",

language = "English",

volume = "98",

pages = "327--336",

journal = "Genomics",

publisher = "Academic Press Inc.",

number = "5",

}

TY - JOUR

T1 - Differential global gene expression in cystic fibrosis nasal and bronchial epithelium

AU - Ogilvie, Varrie

AU - Passmore, Margaret

AU - Hyndman, Laura

AU - Jones, Lisa

AU - Stevenson, Barbara

AU - Wilson, Abigail

AU - Davidson, Heather

AU - Kitchen, Robert R

AU - Gray, Robert D

AU - Shah, Pallav

AU - Alton, Eric W

AU - Davies, Jane C

AU - Porteous, David J

AU - Boyd, A Christopher

PY - 2011

Y1 - 2011

N2 - Respiratory epithelium is the target of therapies, such as gene therapy, for cystic fibrosis (CF) lung disease. To determine the usefulness of the nasal epithelium as a pre-screen for lung-directed therapies, we profiled gene expression in CF and non-CF nasal and bronchial epithelium samples using Illumina HumanRef-8 Expression BeadChips. 863 genes were differentially expressed between CF and non-CF bronchial epithelium but only 15 were differentially expressed between CF and non-CF nasal epithelium (≥1.5-fold, P≤0.05). The most enriched pathway in CF bronchial epithelium was inflammatory response, whereas in CF nasal epithelium it was amino acid metabolism. We also compared nasal and bronchial epithelium in each group and identified differential expression of cellular movement genes in CF patients and cellular growth genes in non-CF subjects. We conclude that CF and non-CF nasal and bronchial epithelium are transcriptionally distinct and CF nasal epithelium is not a good surrogate for the lung respiratory epithelium.

AB - Respiratory epithelium is the target of therapies, such as gene therapy, for cystic fibrosis (CF) lung disease. To determine the usefulness of the nasal epithelium as a pre-screen for lung-directed therapies, we profiled gene expression in CF and non-CF nasal and bronchial epithelium samples using Illumina HumanRef-8 Expression BeadChips. 863 genes were differentially expressed between CF and non-CF bronchial epithelium but only 15 were differentially expressed between CF and non-CF nasal epithelium (≥1.5-fold, P≤0.05). The most enriched pathway in CF bronchial epithelium was inflammatory response, whereas in CF nasal epithelium it was amino acid metabolism. We also compared nasal and bronchial epithelium in each group and identified differential expression of cellular movement genes in CF patients and cellular growth genes in non-CF subjects. We conclude that CF and non-CF nasal and bronchial epithelium are transcriptionally distinct and CF nasal epithelium is not a good surrogate for the lung respiratory epithelium.

KW - Adolescent

KW - Adult

KW - Bronchi

KW - Case-Control Studies

KW - Child

KW - Cystic Fibrosis

KW - Cystic Fibrosis Transmembrane Conductance Regulator

KW - Female

KW - Gene Expression Regulation

KW - Humans

KW - Immunohistochemistry

KW - Inflammation

KW - Keratins

KW - Male

KW - Nasal Mucosa

KW - Oligonucleotide Array Sequence Analysis

KW - Young Adult

UR - http://www.scopus.com/inward/record.url?scp=80055096007&partnerID=8YFLogxK

U2 - 10.1016/j.ygeno.2011.06.008

DO - 10.1016/j.ygeno.2011.06.008

M3 - Article

C2 - 21756994

VL - 98

SP - 327

EP - 336

JO - Genomics

JF - Genomics

IS - 5

ER -

Differential global gene expression in cystic fibrosis nasal and bronchial epithelium

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Cite this