Differential global gene expression in cystic fibrosis nasal and bronchial epithelium

Varrie Ogilvie, Margaret Passmore, Laura Hyndman, Lisa Jones, Barbara Stevenson, Abigail Wilson, Heather Davidson, Robert R Kitchen, Robert D Gray, Pallav Shah, Eric W Alton, Jane C Davies, David J Porteous, A Christopher Boyd

Research output: Contribution to journalArticlepeer-review

Abstract

Respiratory epithelium is the target of therapies, such as gene therapy, for cystic fibrosis (CF) lung disease. To determine the usefulness of the nasal epithelium as a pre-screen for lung-directed therapies, we profiled gene expression in CF and non-CF nasal and bronchial epithelium samples using Illumina HumanRef-8 Expression BeadChips. 863 genes were differentially expressed between CF and non-CF bronchial epithelium but only 15 were differentially expressed between CF and non-CF nasal epithelium (≥1.5-fold, P≤0.05). The most enriched pathway in CF bronchial epithelium was inflammatory response, whereas in CF nasal epithelium it was amino acid metabolism. We also compared nasal and bronchial epithelium in each group and identified differential expression of cellular movement genes in CF patients and cellular growth genes in non-CF subjects. We conclude that CF and non-CF nasal and bronchial epithelium are transcriptionally distinct and CF nasal epithelium is not a good surrogate for the lung respiratory epithelium.
Original languageEnglish
Pages (from-to)327-336
Number of pages10
JournalGenomics
Volume98
Issue number5
DOIs
Publication statusPublished - 2011

Keywords

  • Adolescent
  • Adult
  • Bronchi
  • Case-Control Studies
  • Child
  • Cystic Fibrosis
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Female
  • Gene Expression Regulation
  • Humans
  • Immunohistochemistry
  • Inflammation
  • Keratins
  • Male
  • Nasal Mucosa
  • Oligonucleotide Array Sequence Analysis
  • Young Adult

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