Differential production of cytokines, reactive oxygen and nitrogen by bovine macrophages

Dirk Werling, Jayne Hope, Chris J Howard, Thomas W Jungi

Research output: Contribution to journalArticlepeer-review

Abstract

Toll-like receptors (TLR) have been described as partially sharing signalling pathways but showing unique ligand specificity and tissue distribution. Here, the response of bovine macrophages (Mphi) and dendritic cells (DC), both derived from monocytes, was compared by exposing them to the TLR-specific ligands lipopolysaccharide, poly(I:C)-double-stranded RNA, and CpG-DNA, as well as inactivated Gram-negative and Gram-positive bacteria, shown to bind to TLR. The production of NO, superoxide anion, interleukin-10 (IL-10), IL-12 and tumour necrosis factor (TNF) was determined. Compared to monocytes, Mphi expressed more TLR2 and similar levels of TLR4 mRNA transcripts, as analysed by quantitative polymerase chain reaction, whereas DC expressed reduced amounts. Although both DC and Mphi recognized the TLR ligands, dramatic differences were seen in their reaction pattern to them. Both cell types responded with the production of TNF, but DC produced more IL-12, whereas Mphi produced more IL-10, regardless of the TLR agonist used. Co-stimulation with interferon-gamma influenced the amount of cytokine production, but did not alter the cell type-specific response pattern. Compared to Mphi, DC produced > 10 times less NO upon triggering with TLR ligands. In addition, DC produced superoxide anion to opsonized and non-opsonized zymosan, but not to phorbol 12-myristate 13-acetate, a response pattern confirmed for human Mphi and DC, respectively. Different protein kinase C isoforms and extracellular signal-regulated kinase patterns were detected in cell lysates of resting and stimulated Mphi and DC. Collectively, our results point to profound differences in pathogen-derived signal-response coupling occurring commensurate with distinct functions carried out by Mphi or DC.
Original languageEnglish
Pages (from-to)41-52
Number of pages10
JournalImmunology
Volume111
Issue number1
DOIs
Publication statusPublished - Jan 2004

Keywords

  • Animals
  • Bacteria
  • Cattle
  • Cells, Cultured
  • CpG Islands
  • Cytokines/biosynthesis
  • DNA/administration & dosage
  • Dendritic Cells/immunology
  • Dendritic Cells/metabolism
  • Interleukin-10/biosynthesis
  • Interleukin-12/biosynthesis
  • Lipopolysaccharides/pharmacology
  • Macrophages/immunology
  • Macrophages/metabolism
  • Membrane Glycoproteins/drug effects
  • Membrane Glycoproteins/metabolism
  • Mitogen-Activated Protein Kinases/metabolism
  • Nitric Oxide/analysis
  • Nitrogen/metabolism
  • Poly I-C/genetics
  • Protein Kinase C/metabolism
  • Reactive Oxygen Species/metabolism
  • Receptors, Cell Surface/drug effects
  • Receptors, Cell Surface/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Superoxides/analysis
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors
  • Tumor Necrosis Factor-alpha/biosynthesis

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