Differential transcription of the bovine AMPD3 gene in single blastocysts cultured under various oxygen tensions

Y. Q. Yuan; L. J. Peelman; J. L. Williams; A. Van Zeveren; A. De Kruif; A. Law; A. Van Soom

doi:10.1111/j.1439-0388.2004.00469.x

Differential transcription of the bovine AMPD3 gene in single blastocysts cultured under various oxygen tensions

Y. Q. Yuan, L. J. Peelman^*, J. L. Williams, A. Van Zeveren, A. De Kruif, A. Law, A. Van Soom

^*Corresponding author for this work

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Studying gene expression during the early embryonic stages of in vitro culture is essential and of special interest, not only to gain insights in embryo development but also to find candidate genes that can be used as markers to assess embryo quality. Using differential display RT-PCR expression of AMPD3 coding for AMP deaminase 3 was found in bovine blastocysts cultured in vitro only under 2% but not under 5 and 20% oxygen tensions. Mapping of this gene to bovine chromosome 15 confirmed its identity. The differential expression of bovine AMPD3 was confirmed by RT-PCR with specific primers.

Original language	English
Pages (from-to)	356-360
Number of pages	5
Journal	Journal of Animal Breeding and Genetics
Volume	121
Issue number	5
DOIs	https://doi.org/10.1111/j.1439-0388.2004.00469.x
Publication status	Published - 13 Sept 2004

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title = "Differential transcription of the bovine AMPD3 gene in single blastocysts cultured under various oxygen tensions",

abstract = "Studying gene expression during the early embryonic stages of in vitro culture is essential and of special interest, not only to gain insights in embryo development but also to find candidate genes that can be used as markers to assess embryo quality. Using differential display RT-PCR expression of AMPD3 coding for AMP deaminase 3 was found in bovine blastocysts cultured in vitro only under 2\% but not under 5 and 20\% oxygen tensions. Mapping of this gene to bovine chromosome 15 confirmed its identity. The differential expression of bovine AMPD3 was confirmed by RT-PCR with specific primers.",

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doi = "10.1111/j.1439-0388.2004.00469.x",

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AU - Yuan, Y. Q.

AU - Peelman, L. J.

AU - Williams, J. L.

AU - Van Zeveren, A.

AU - De Kruif, A.

AU - Law, A.

AU - Van Soom, A.

PY - 2004/9/13

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AB - Studying gene expression during the early embryonic stages of in vitro culture is essential and of special interest, not only to gain insights in embryo development but also to find candidate genes that can be used as markers to assess embryo quality. Using differential display RT-PCR expression of AMPD3 coding for AMP deaminase 3 was found in bovine blastocysts cultured in vitro only under 2% but not under 5 and 20% oxygen tensions. Mapping of this gene to bovine chromosome 15 confirmed its identity. The differential expression of bovine AMPD3 was confirmed by RT-PCR with specific primers.

UR - https://www.scopus.com/pages/publications/4644237687

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JO - Journal of Animal Breeding and Genetics

JF - Journal of Animal Breeding and Genetics

IS - 5

ER -

Differential transcription of the bovine AMPD3 gene in single blastocysts cultured under various oxygen tensions

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