Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification

M. M. Taema, B. C. Maddison, L. Thorne, K. Bishop, J. Owen, N. Hunter, C. A. Baker, L. A. Terry, K. C. Gough

Research output: Contribution to journalArticlepeer-review

Abstract

Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V(136)R(154)Q(171)/V(136)R(154)Q(171) or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.
Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalMolecular Biotechnology
Volume51
Issue number3
DOIs
Publication statusPublished - Jul 2012

Keywords

  • scrapie
  • prion
  • strain
  • BSE
  • CH1641

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