Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification

M. M. Taema; B. C. Maddison; L. Thorne; K. Bishop; J. Owen; N. Hunter; C. A. Baker; L. A. Terry; K. C. Gough

doi:10.1007/s12033-011-9460-0

Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification

M. M. Taema, B. C. Maddison, L. Thorne, K. Bishop, J. Owen, N. Hunter, C. A. Baker, L. A. Terry, K. C. Gough

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V(136)R(154)Q(171)/V(136)R(154)Q(171) or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.

Original language	English
Pages (from-to)	233-239
Number of pages	7
Journal	Molecular Biotechnology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1007/s12033-011-9460-0
Publication status	Published - Jul 2012

Keywords / Materials (for Non-textual outputs)

scrapie
prion
strain
BSE
CH1641

Access to Document

10.1007/s12033-011-9460-0

http://www.springerlink.com/content/p983k24j25414h15/?MUD=MP

Livestock neurobiology
Gill, A., Barron, R., Beard, P., Brunton, P., Goldmann, W., Hume, D., Hunter, N., Lawrence, A., Mabbott, N., Manson, J., McColl, B., Meddle, S. & Wishart, T.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

@article{b04b7d5d5a8c499b8866fa7e9e17dece,

title = "Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification",

abstract = "Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V(136)R(154)Q(171)/V(136)R(154)Q(171) or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.",

keywords = "scrapie , prion , strain , BSE , CH1641",

author = "Taema, {M. M.} and Maddison, {B. C.} and L. Thorne and K. Bishop and J. Owen and N. Hunter and Baker, {C. A.} and Terry, {L. A.} and Gough, {K. C.}",

note = "Times Cited: 0 Taema, Maged M. Maddison, Ben C. Thorne, Leigh Bishop, Keith Owen, Jonathan Hunter, Nora Baker, Claire A. Terry, Linda A. Gough, Kevin C.",

year = "2012",

month = jul,

doi = "10.1007/s12033-011-9460-0",

language = "English",

volume = "51",

pages = "233--239",

journal = "Molecular Biotechnology",

publisher = "Humana Press",

number = "3",

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TY - JOUR

T1 - Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification

AU - Taema, M. M.

AU - Maddison, B. C.

AU - Thorne, L.

AU - Bishop, K.

AU - Owen, J.

AU - Hunter, N.

AU - Baker, C. A.

AU - Terry, L. A.

AU - Gough, K. C.

N1 - Times Cited: 0 Taema, Maged M. Maddison, Ben C. Thorne, Leigh Bishop, Keith Owen, Jonathan Hunter, Nora Baker, Claire A. Terry, Linda A. Gough, Kevin C.

PY - 2012/7

Y1 - 2012/7

N2 - Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V(136)R(154)Q(171)/V(136)R(154)Q(171) or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.

AB - Whilst ovine BSE displays distinct pathological characteristics to ovine CH1641-like scrapie upon passage in rodents, they have very similar molecular phenotypes. As such, the in vitro differentiation of these strains in routine surveillance programmes presents a significant diagnostic challenge. In this study, using serial protein-misfolding cyclic amplification (sPMCA), ovine BSE was readily amplified in vitro in brain substrates from sheep with V(136)R(154)Q(171)/V(136)R(154)Q(171) or AHQ/AHQ PRNP genotypes. In contrast, the CH1641 strain was refractory to such amplification. This method allowed for complete and unequivocal differentiation of experimental BSE from CH1641 prion strains within an ovine host.

KW - scrapie

KW - prion

KW - strain

KW - BSE

KW - CH1641

U2 - 10.1007/s12033-011-9460-0

DO - 10.1007/s12033-011-9460-0

M3 - Article

VL - 51

SP - 233

EP - 239

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 3

ER -

Differentiating Ovine BSE from CH1641 Scrapie by Serial Protein Misfolding Cyclic Amplification

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Livestock neurobiology

Cite this