Differentiation antigens on bovine mononuclear phagocytes identified by monoclonal antibodies

J A Ellis, W.C. Davis, N D MacHugh, D.L. Emery, A Kaushal, Ivan Morrison

Research output: Contribution to journalArticlepeer-review

Abstract

Five monoclonal antibodies (MAb) produced against cell surface antigens on bovine mononuclear phagocytes (MPh) were characterized. None of the MAb recognized erythrocytes, thrombocytes, B lymphocytes or resting or activated T lymphocytes. Two MAb (IL-A22 and IL-A24) reacted with the majority of monocytes and granulocytes in peripheral blood, with 20-40% bone marrow cells comprising myelo-monocytic cells, and with a proportion of mature macrophages. Reactivity of the remaining three MAb was restricted to MPh: one of these (IL-A25) was apparently specific for pulmonary macrophages, whereas the molecules recognized by the other two (IL-A23 and CH16A) were expressed on subpopulations of blood monocytes and tissue macrophages. None of the MAb inhibited adherence of MPh to plasma-coated gelating surfaces or Fc-mediated rosette formation. One of the MAb, IL-A24, which reacts with MPh and granulocytes, inhibited antigen-specific proliferative response or peripheral blood mononuclear leukocytes (PBM) to the soluble antigen, keyhole limpet hemocyanin (KLH) but did not inhibit responses to concanavalin A or allogeneic leukocytes. This MAb was shown to react with two polypeptides of approximately 75 kD and 110 kD on the surface of peripheral blood monocytes.
Original languageEnglish
Pages (from-to)325-40
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume19
Issue number3-4
Publication statusPublished - Oct 1988

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation/immunology
  • Cattle/immunology
  • Female
  • Leukocytes, Mononuclear/immunology
  • Lung/immunology
  • Macrophages/immunology
  • Phagocytes/classification
  • Phagocytes/immunology

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