Differentiation of mouse embryonic stem cells to neuronal cells using hanging droplets and retinoic acid

Jeroen Witteveldt; Sara Macias Ribela

doi:10.21769/BioProtoc.3417

Differentiation of mouse embryonic stem cells to neuronal cells using hanging droplets and retinoic acid

Jeroen Witteveldt, Sara Macias Ribela

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.

Original language	English
Journal	Bio-protocol
Volume	9
Issue number	21
Early online date	5 Nov 2019
DOIs	https://doi.org/10.21769/BioProtoc.3417
Publication status	E-pub ahead of print - 5 Nov 2019

Keywords / Materials (for Non-textual outputs)

embryonic stem cells
embryoid bodies
retinoic acid
hanging droplets
differentiation
neuronal

Access to Document

10.21769/BioProtoc.3417Licence: Creative Commons: Attribution (CC-BY)

MaciasEtalBP2019DifferentiationOfMouseEmbryonicStemCellsToNeuronalCellsFinal published version, 946 KBLicence: Creative Commons: Attribution (CC-BY)

Antiviral defence mechanisms: small RNAs versus Interferon pathway
Macias Ribela, S.
UK-based charities
1/01/16 → 31/12/21
Project: Research

Cite this

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title = "Differentiation of mouse embryonic stem cells to neuronal cells using hanging droplets and retinoic acid",

abstract = "Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.",

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author = "Jeroen Witteveldt and {Macias Ribela}, Sara",

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AU - Macias Ribela, Sara

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AB - Controlled differentiation of embryonic stem cells is an essential tool in stem cell research. In this protocol, we describe a simple differentiation protocol involving the induction of embryoid body formation in mouse embryonic stem cells (mESC) using hanging droplets, followed by differentiation into a neuronal lineage.

KW - embryonic stem cells

KW - embryoid bodies

KW - retinoic acid

KW - hanging droplets

KW - differentiation

KW - neuronal

U2 - 10.21769/BioProtoc.3417

DO - 10.21769/BioProtoc.3417

M3 - Article

SN - 2331-8325

VL - 9

JO - Bio-protocol

JF - Bio-protocol

IS - 21

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Differentiation of mouse embryonic stem cells to neuronal cells using hanging droplets and retinoic acid

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Antiviral defence mechanisms: small RNAs versus Interferon pathway

Cite this