Dimensions and Global Twist of Single-Layer DNA Origami Measured by Small-Angle X-ray Scattering

Matthew A.B. Baker, Andrew J. Tuckwell, Jonathan F. Berengut, Jonathan Bath, Florence Benn, Anthony P. Duff, Andrew E. Whitten, Katherine E. Dunn, Robert M. Hynson, Andrew J. Turberfield, Lawrence K. Lee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The rational design of complementary DNA sequences can be used to create nanostructures that self-assemble with nanometer precision. DNA nanostructures have been imaged by atomic force microscopy and electron microscopy. Small-angle X-ray scattering (SAXS) provides complementary structural information on the ensemble-averaged state of DNA nanostructures in solution. Here we demonstrate that SAXS can distinguish between different single-layer DNA origami tiles that look identical when immobilized on a mica surface and imaged with atomic force microscopy. We use SAXS to quantify the magnitude of global twist of DNA origami tiles with different crossover periodicities: these measurements highlight the extreme structural sensitivity of single-layer origami to the location of strand crossovers. We also use SAXS to quantify the distance between pairs of gold nanoparticles tethered to specific locations on a DNA origami tile and use this method to measure the overall dimensions and geometry of the DNA nanostructure in solution. Finally, we use indirect Fourier methods, which have long been used for the interpretation of SAXS data from biomolecules, to measure the distance between DNA helix pairs in a DNA origami nanotube. Together, these results provide important methodological advances in the use of SAXS to analyze DNA nanostructures in solution and insights into the structures of single-layer DNA origami.

Original languageEnglish
Pages (from-to)5791-5799
Number of pages9
JournalACS Nano
Volume12
Issue number6
Early online date29 May 2018
DOIs
Publication statusPublished - 26 Jun 2018

Keywords

  • DNA nanotechnology
  • DNA origami
  • DNA self-assembly
  • gold nanoparticles
  • small-angle X-ray scattering

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