Projects per year
Abstract
Amino acids are key synthetic building blocks that can be prepared in an enantiopure form by biocatalytic methods. We show that the L-selective ornithine deacetylase ArgE catalyses hydrolysis of a wide-range of N-acyl-amino acid substrates. This activity was revealed by 1H NMR spectroscopy that monitored the appearance of the well resolved signal of the acetate product. Furthermore, the assay was used to probe the subtle structural selectivity of the biocatalyst using a substrate that could adopt different rotameric conformations.
Original language | English |
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Number of pages | 6 |
Journal | Organic & Biomolecular chemistry |
Early online date | 3 May 2021 |
DOIs | |
Publication status | E-pub ahead of print - 3 May 2021 |
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Dive into the research topics of 'Direct monitoring of biocatalytic deacetylation of amino acid substrates by 1H NMR reveals fine details of substrate specificity'. Together they form a unique fingerprint.Projects
- 1 Finished
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A National Network for Applications of High-Field NMR in the Life and Physical Sciences
Uhrin, D. (Principal Investigator), Barlow, P. (Co-investigator), Bell, N. (Co-investigator) & Bramham, J. (Co-investigator)
1/05/18 → 30/04/21
Project: Research