Projects per year
Abstract / Description of output
CENP-A chromatin specifies mammalian centromere identity, and its chaperone HJURP replenishes CENP-A when recruited by the Mis18 complex (Mis18C) via M18BP1/KNL2 to CENP-C at kinetochores during interphase. However, the Mis18C recruitment mechanism remains unresolved in species lacking M18BP1, such as fission yeast. Fission yeast centromeres cluster at G2 spindle pole bodies (SPBs) when CENPACnp1 is replenished and where Mis18C also localizes. We show that SPBs play an unexpected role in concentrating Mis18C near centromeres through the recruitment of Mis18 by direct binding to the major SPB linker of nucleoskeleton and cytoskeleton (LINC) component Sad1. Mis18C recruitment by Sad1 is important for CENP-ACnp1 chromatin establishment and acts in parallel with a CENP-C-mediated Mis18C recruitment pathway to maintain centromeric CENP-ACnp1 but operates independently of Sad1-mediated centromere clustering. SPBs therefore provide a non-chromosomal scaffold for both Mis18C recruitment and centromere clustering during G2. This centromere-independent Mis18-SPB recruitment provides a mechanism that governs de novo CENP-ACnp1 chromatin assembly by the proximity of appropriate sequences to SPBs and highlights how nuclear spatial organization influences centromere identity.
Original language | English |
---|---|
Pages (from-to) | 4187-4201 |
Number of pages | 22 |
Journal | Current Biology |
Volume | 33 |
Issue number | 19 |
Early online date | 14 Sept 2023 |
DOIs | |
Publication status | Published - 9 Oct 2023 |
Keywords / Materials (for Non-textual outputs)
- centromere
- Mis18
- CENP-A
- spindle pole
- nucleus
- Sad1
- chromatin
- Kinetochore
- S. pombe
Fingerprint
Dive into the research topics of 'Direct recruitment of Mis18 to interphase spindle pole bodies promotes CENP-A chromatin assembly'. Together they form a unique fingerprint.-
-
-
Epigenetic inheritance: establishment and transmission of specialised chromatin domains
1/04/17 → 31/03/23
Project: Research