Direct sequencing of small genomes on the Pacific Biosciences RS without library preparation

Paul Coupland, Tamir Chandra, Mike Quail, Wolf Reik, Harold Swerdlow

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We have developed a sequencing method on the Pacific Biosciences RS sequencer (the PacBio) for small DNA molecules that avoids the need for a standard library preparation. To date this approach has been applied toward sequencing single-stranded and double-stranded viral genomes, bacterial plasmids, plasmid vector models for DNA-modification analysis, and linear DNA fragments covering an entire bacterial genome. Using direct sequencing it is possible to generate sequence data from as little as 1 ng of DNA, offering a significant advantage over current protocols which typically require 400-500 ng of sheared DNA for the library preparation.

Original languageEnglish
Pages (from-to)365-72
Number of pages8
JournalBiotechniques
Volume53
Issue number6
DOIs
Publication statusPublished - Dec 2012

Keywords / Materials (for Non-textual outputs)

  • Base Sequence
  • Gene Library
  • Genome, Bacterial
  • Genomics
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Software
  • User-Computer Interface

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