Discrimination between major histocompatibility complex class II DQ and DR locus products in cattle

B M Dutia, L MacCarthy-Morrogh, E J Glass, G Knowles, R L Spooner, J Hopkins

Research output: Contribution to journalArticlepeer-review

Abstract

We have used a panel of anti-major histocompatibility complex (MHC) class II monoclonal antibodies (mAbs) and have assessed their specificity for the products of the individual bovine MHC (BoLA) class II subregions. The mAbs identified two distinct class II molecules by affinity purification and ELISA. Two-dimensional immunoblotting confirmed these data and NH2-terminal sequencing of the purified class II alpha chains of one member of each group identified the subregion specificity of the mAbs. The mAbs VPM36, TH22A and TH81A are specific for BoLA DQ, whereas VPM54, TH14B and J11 are specific for BoLA DR. SW73.2 reacts with both MHC subgroups of all cattle tested.
Original languageEnglish
Pages (from-to)111-4
Number of pages4
JournalAnimal Genetics
Volume26
Issue number2
Publication statusPublished - Apr 1995

Keywords

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Cattle
  • Histocompatibility Antigens Class II
  • Humans
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid

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