Disposable electrochemical printed gold chips for the analysis of acetylcholinesterase inhibition

Vladimir Dounin; Anthony J Veloso; Holger Schulze; Till T Bachmann; Kagan Kerman

doi:10.1016/j.aca.2010.04.037

Disposable electrochemical printed gold chips for the analysis of acetylcholinesterase inhibition

Vladimir Dounin, Anthony J Veloso, Holger Schulze, Till T Bachmann, Kagan Kerman

Research output: Contribution to journal › Article › peer-review

Abstract

The detection of trace levels of paraoxon and carbofuran was achieved utilizing differential pulse voltammetry (DPV) on gold disposable electrochemical printed (DEP) chips. The nanostructured gold surface of the chips enables highly sensitive oxidation of the thiocholine (TCh) product even in the absence of costly surface modifications. The inhibition of AChE activity at varying insecticide concentrations was detected with low detection limits of 10 ppb (36 nM) for paraoxon and 8 ppb (18 nM) for carbofuran. Fine-tuning of the experimental conditions will allow for the application of unmodified DEP gold chips for inexpensive on-field detection of AChE inhibition by various insecticides at or below the allowable concentrations set by European and North American regulation standards.

Original language	English
Pages (from-to)	63-7
Number of pages	5
Journal	Analytica Chimica Acta
Volume	669
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aca.2010.04.037
Publication status	Published - 2010

Keywords / Materials (for Non-textual outputs)

Carbofuran
Cholinesterase Inhibitors
Electrochemistry
Gold
Insecticides
Limit of Detection
Nanostructures
Paraoxon
Thiocholine

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10.1016/j.aca.2010.04.037

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title = "Disposable electrochemical printed gold chips for the analysis of acetylcholinesterase inhibition",

abstract = "The detection of trace levels of paraoxon and carbofuran was achieved utilizing differential pulse voltammetry (DPV) on gold disposable electrochemical printed (DEP) chips. The nanostructured gold surface of the chips enables highly sensitive oxidation of the thiocholine (TCh) product even in the absence of costly surface modifications. The inhibition of AChE activity at varying insecticide concentrations was detected with low detection limits of 10 ppb (36 nM) for paraoxon and 8 ppb (18 nM) for carbofuran. Fine-tuning of the experimental conditions will allow for the application of unmodified DEP gold chips for inexpensive on-field detection of AChE inhibition by various insecticides at or below the allowable concentrations set by European and North American regulation standards.",

keywords = "Carbofuran, Cholinesterase Inhibitors, Electrochemistry, Gold, Insecticides, Limit of Detection, Nanostructures, Paraoxon, Thiocholine",

author = "Vladimir Dounin and Veloso, {Anthony J} and Holger Schulze and Bachmann, {Till T} and Kagan Kerman",

year = "2010",

doi = "10.1016/j.aca.2010.04.037",

language = "English",

volume = "669",

pages = "63--7",

journal = "Analytica Chimica Acta",

issn = "0003-2670",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Disposable electrochemical printed gold chips for the analysis of acetylcholinesterase inhibition

AU - Dounin, Vladimir

AU - Veloso, Anthony J

AU - Schulze, Holger

AU - Bachmann, Till T

AU - Kerman, Kagan

PY - 2010

Y1 - 2010

N2 - The detection of trace levels of paraoxon and carbofuran was achieved utilizing differential pulse voltammetry (DPV) on gold disposable electrochemical printed (DEP) chips. The nanostructured gold surface of the chips enables highly sensitive oxidation of the thiocholine (TCh) product even in the absence of costly surface modifications. The inhibition of AChE activity at varying insecticide concentrations was detected with low detection limits of 10 ppb (36 nM) for paraoxon and 8 ppb (18 nM) for carbofuran. Fine-tuning of the experimental conditions will allow for the application of unmodified DEP gold chips for inexpensive on-field detection of AChE inhibition by various insecticides at or below the allowable concentrations set by European and North American regulation standards.

AB - The detection of trace levels of paraoxon and carbofuran was achieved utilizing differential pulse voltammetry (DPV) on gold disposable electrochemical printed (DEP) chips. The nanostructured gold surface of the chips enables highly sensitive oxidation of the thiocholine (TCh) product even in the absence of costly surface modifications. The inhibition of AChE activity at varying insecticide concentrations was detected with low detection limits of 10 ppb (36 nM) for paraoxon and 8 ppb (18 nM) for carbofuran. Fine-tuning of the experimental conditions will allow for the application of unmodified DEP gold chips for inexpensive on-field detection of AChE inhibition by various insecticides at or below the allowable concentrations set by European and North American regulation standards.

KW - Carbofuran

KW - Cholinesterase Inhibitors

KW - Electrochemistry

KW - Gold

KW - Insecticides

KW - Limit of Detection

KW - Nanostructures

KW - Paraoxon

KW - Thiocholine

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U2 - 10.1016/j.aca.2010.04.037

DO - 10.1016/j.aca.2010.04.037

M3 - Article

C2 - 20510904

SN - 0003-2670

VL - 669

SP - 63

EP - 67

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1-2

ER -

Disposable electrochemical printed gold chips for the analysis of acetylcholinesterase inhibition

Abstract

Keywords / Materials (for Non-textual outputs)

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