Distinct sources and targets of IL-10 during dendritic cell-driven Th1 and Th2 responses in vivo

Georgia Perona-Wright; Stephen J Jenkins; Alison Crawford; David Gray; Edward J Pearce; Andrew S MacDonald

doi:10.1002/eji.200535722

Distinct sources and targets of IL-10 during dendritic cell-driven Th1 and Th2 responses in vivo

Georgia Perona-Wright, Stephen J Jenkins, Alison Crawford, David Gray, Edward J Pearce, Andrew S MacDonald

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Dendritic cells (DC) can both initiate an immune response and dictate its character. Cytokines are critically involved in this process and, although interleukin (IL)-10 is known as a potent immunosuppressant, the impact of its release from DC remains unclear. Here, we transfer pathogen-conditioned murine DC in vivo and show that, while DC-derived IL-10 can act to limit Th1 development, it is not required for Th2 induction. In both Th2 and Th1 settings, however, IL-10 from cells other than the initiating DC dominates the regulation of the emerging effector cell populations. Surprisingly, the critical source of IL-10 in this process is neither T nor B cells. These data illustrate the distinct actions of IL-10 during differently polarised, pathogen-focussed, DC-driven immune responses in vivo.

Original language	English
Pages (from-to)	2367-75
Number of pages	9
Journal	European Journal of Immunology
Volume	36
Issue number	9
DOIs	https://doi.org/10.1002/eji.200535722
Publication status	Published - 2006

Keywords / Materials (for Non-textual outputs)

Animals
Antigen Presentation
Antigens, Bacterial
Antigens, Helminth
B-Lymphocytes
Dendritic Cells
Interleukin-10
Lymphocyte Activation
Mice
Th1 Cells
Th2 Cells

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10.1002/eji.200535722

Cite this

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title = "Distinct sources and targets of IL-10 during dendritic cell-driven Th1 and Th2 responses in vivo",

abstract = "Dendritic cells (DC) can both initiate an immune response and dictate its character. Cytokines are critically involved in this process and, although interleukin (IL)-10 is known as a potent immunosuppressant, the impact of its release from DC remains unclear. Here, we transfer pathogen-conditioned murine DC in vivo and show that, while DC-derived IL-10 can act to limit Th1 development, it is not required for Th2 induction. In both Th2 and Th1 settings, however, IL-10 from cells other than the initiating DC dominates the regulation of the emerging effector cell populations. Surprisingly, the critical source of IL-10 in this process is neither T nor B cells. These data illustrate the distinct actions of IL-10 during differently polarised, pathogen-focussed, DC-driven immune responses in vivo.",

keywords = "Animals, Antigen Presentation, Antigens, Bacterial, Antigens, Helminth, B-Lymphocytes, Dendritic Cells, Interleukin-10, Lymphocyte Activation, Mice, Th1 Cells, Th2 Cells",

author = "Georgia Perona-Wright and Jenkins, {Stephen J} and Alison Crawford and David Gray and Pearce, {Edward J} and MacDonald, {Andrew S}",

year = "2006",

doi = "10.1002/eji.200535722",

language = "English",

volume = "36",

pages = "2367--75",

journal = "European Journal of Immunology",

issn = "1521-4141",

publisher = "Wiley-VCH",

number = "9",

}

TY - JOUR

T1 - Distinct sources and targets of IL-10 during dendritic cell-driven Th1 and Th2 responses in vivo

AU - Perona-Wright, Georgia

AU - Jenkins, Stephen J

AU - Crawford, Alison

AU - Gray, David

AU - Pearce, Edward J

AU - MacDonald, Andrew S

PY - 2006

Y1 - 2006

N2 - Dendritic cells (DC) can both initiate an immune response and dictate its character. Cytokines are critically involved in this process and, although interleukin (IL)-10 is known as a potent immunosuppressant, the impact of its release from DC remains unclear. Here, we transfer pathogen-conditioned murine DC in vivo and show that, while DC-derived IL-10 can act to limit Th1 development, it is not required for Th2 induction. In both Th2 and Th1 settings, however, IL-10 from cells other than the initiating DC dominates the regulation of the emerging effector cell populations. Surprisingly, the critical source of IL-10 in this process is neither T nor B cells. These data illustrate the distinct actions of IL-10 during differently polarised, pathogen-focussed, DC-driven immune responses in vivo.

AB - Dendritic cells (DC) can both initiate an immune response and dictate its character. Cytokines are critically involved in this process and, although interleukin (IL)-10 is known as a potent immunosuppressant, the impact of its release from DC remains unclear. Here, we transfer pathogen-conditioned murine DC in vivo and show that, while DC-derived IL-10 can act to limit Th1 development, it is not required for Th2 induction. In both Th2 and Th1 settings, however, IL-10 from cells other than the initiating DC dominates the regulation of the emerging effector cell populations. Surprisingly, the critical source of IL-10 in this process is neither T nor B cells. These data illustrate the distinct actions of IL-10 during differently polarised, pathogen-focussed, DC-driven immune responses in vivo.

KW - Animals

KW - Antigen Presentation

KW - Antigens, Bacterial

KW - Antigens, Helminth

KW - B-Lymphocytes

KW - Dendritic Cells

KW - Interleukin-10

KW - Lymphocyte Activation

KW - Mice

KW - Th1 Cells

KW - Th2 Cells

U2 - 10.1002/eji.200535722

DO - 10.1002/eji.200535722

M3 - Article

C2 - 16917957

SN - 1521-4141

VL - 36

SP - 2367

EP - 2375

JO - European Journal of Immunology

JF - European Journal of Immunology

IS - 9

ER -

Distinct sources and targets of IL-10 during dendritic cell-driven Th1 and Th2 responses in vivo

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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