Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells

Anne E Wiblin, Wei Cui, A John Clark, Wendy A Bickmore

Research output: Contribution to journalArticlepeer-review

Abstract

Nuclear organisation is thought to be important in regulating gene expression. Here we investigate whether human embryonic stem cells (hES) have a particular nuclear organisation, which could be important for maintaining their pluripotent state. We found that whereas the nuclei of hES cells have a general gene-density-related radial organisation of chromosomes, as is seen in differentiated cells, there are also distinctive localisations for chromosome regions and gene loci with a role in pluripotency. Chromosome 12p, a region of the human genome that contains clustered pluripotency genes including NANOG, has a more central nuclear localisation in ES cells than in differentiated cells. On chromosome 6p we find no overall change in nuclear chromosome position, but instead we detect a relocalisation of the OCT4 locus, to a position outside its chromosome territory. There is also a smaller proportion of centromeres located close to the nuclear periphery in hES cells compared to differentiated cells. We conclude that hES cell nuclei have a distinct nuclear architecture, especially at loci involved in maintaining pluripotency. Understanding this level of hES cell biology provides a framework within which other large-scale chromatin changes that may accompany differentiation can be considered.
Original languageEnglish
Pages (from-to)3861-8
Number of pages8
JournalJournal of Cell Science
Volume118
Issue numberPt 17
DOIs
Publication statusPublished - 2005

Fingerprint

Dive into the research topics of 'Distinctive nuclear organisation of centromeres and regions involved in pluripotency in human embryonic stem cells'. Together they form a unique fingerprint.

Cite this