Division site selection protein DivIVA of Bacillus subtilis has a second distinct function in chromosome segregation during sporulation

H B Thomaides, M Freeman, M El Karoui, J Errington

Research output: Contribution to journalArticlepeer-review

Abstract

DivIVA is a coiled-coil, tropomyosin-like protein of Gram-positive bacteria. Previous work showed that this protein is targeted to division sites and retained at the cell poles after division. In vegetative cells, DivIVA sequesters the MinCD division inhibitor to the cell poles, thereby helping to direct cell division to the correct midcell site. We now show that DivIVA has a second, quite separate role in sporulating cells of Bacillus subtilis. It again acts at the cell pole but in this case interacts with the chromosome segregation machinery to help position the oriC region of the chromosome at the cell pole, in preparation for polar division. We isolated mutations in divIVA that separate the protein's role in sporulation from its vegetative function in cell division. DivIVA therefore appears to be a bifunctional protein with distinct roles in division-site selection and chromosome segregation.
Original languageEnglish
Pages (from-to)1662-73
Number of pages12
JournalGenes & Development
Volume15
Issue number13
DOIs
Publication statusPublished - 2001

Keywords

  • Bacillus subtilis
  • chromosome segregation
  • divIVA gene
  • sporulation
  • minicell

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