DNA barcoding of European Herbertus (Marchantiopsida, Herbertaceae) and the discovery and description of a new species

David Bell, David G Long, Alan D Forrest, Michelle L Hollingsworth, Hans H Blom, Peter M Hollingsworth

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

DNA barcoding of a group of European liverwort species from the genus Herbertus was undertaken using three plastid (matK, rbcL and trnH-psbA) and one nuclear (ITS) marker. The DNA barcode data were effective in discriminating among the sampled species of Herbertus and contributed towards the detection of a previously overlooked European Herbertus species, described here as H. norenus sp. nov. This species shows clear-cut differences in DNA sequence for multiple barcode regions and is also morphologically distinct. The DNA barcode data were also useful in clarifying taxonomic relationships of the European species with some species from Asia and North America. In terms of the discriminatory power of the different barcode markers, ITS was the most informative region, followed closely by matK. All species were distinguishable by ITS alone, rbcL + matK and various other multimarker combinations.
Original languageEnglish
Pages (from-to)36-47
Number of pages12
JournalMolecular Ecology Resources
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords / Materials (for Non-textual outputs)

  • DNA Barcoding, Taxonomic
  • DNA, Plant
  • Europe
  • Hepatophyta
  • Molecular Sequence Data
  • Phylogeny
  • Plastids

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