Projects per year
Abstract
The DNA hypomethylation that occurs when embryonic stem cells (ESCs) are directed to the ground state of naive pluripotency by culturing in two small molecule inhibitors (2i) results in redistribution of polycomb (H3K27me3) away from its target loci. Here, we demonstrate that 3D genome organization is also altered in 2i, with chromatin decompaction at polycomb target loci and a loss of long-range polycomb interactions. By preventing DNA hypomethylation during the transition to the ground state, we are able to restore to ESC in 2i the H3K27me3 distribution, as well as polycomb-mediated 3D genome organization that is characteristic of primed ESCs grown in serum. However, these cells retain the functional characteristics of 2i ground-state ESCs. Our findings demonstrate the central role of DNA methylation in shaping major aspects of 3D genome organization but caution against assuming causal roles for the epigenome and 3D genome in gene regulation and function in ESCs.
Original language | English |
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Pages (from-to) | 1974-1985.e6 |
Number of pages | 18 |
Journal | Cell Reports |
Volume | 29 |
Issue number | 7 |
DOIs | |
Publication status | Published - 12 Nov 2019 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Chromatin/genetics
- Chromatin Assembly and Disassembly
- DNA Methylation
- Epigenome
- Male
- Mice
- Mice, Knockout
- Mouse Embryonic Stem Cells/cytology
Fingerprint
Dive into the research topics of 'DNA Methylation Directs Polycomb-Dependent 3D Genome Re-organization in Naive Pluripotency'. Together they form a unique fingerprint.Projects
- 2 Finished
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MC_UU_00007/2 The role of spatial nuclear organisation in genome function
1/04/18 → 1/04/23
Project: Research
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Maintaining Genetic and Chromosomal Stability in the Mammalian Germline
1/04/18 → 31/03/23
Project: Research
Profiles
-
Ian Adams
- Deanery of Molecular, Genetic and Population Health Sciences - Personal Chair of Germline Biology
- MRC Human Genetics Unit
- Royal (Dick) School of Veterinary Studies - Chair position in Reproductive Biotechnologies
Person: Academic: Research Active