DNA microarray for the detection of therapeutically relevant antibiotic resistance determinants in clinical isolates of Staphylococcus aureus

Birgit Strommenger, Christiane Schmidt, Guido Werner, Beate Roessle-Lorch, Till T Bachmann, Wolfgang Witte

Research output: Contribution to journalArticlepeer-review

Abstract

An oligonucleotide microarray was constructed for the rapid and sensitive molecular detection of antibiotic resistance determinants in Staphylococcus aureus. The array is equipped with oligonucleotide capture probes for the detection of 10 clinically and therapeutically relevant antibiotic resistance genes and -mutations (mecA, aacA-aphD, tetK, tetM, vat(A), vat(B), vat(C), erm(A), erm(C), grlA-mutation) as well as several control probes. A microarray concept was established including multiplexed PCR amplification, DNA labeling, hybridization and data processing. This concept was applied to clinical Staphylococcus aureus isolates and results were concordant with those from standard genotypic and phenotypic resistance testing. Our microarray concept offers rapid and accurate identification of antibiotic resistance profiles. It is easily expandable and thus can be adapted to changing clinical and epidemiological requirements in clinical diagnosis as well as in epidemiological studies.
Original languageEnglish
Pages (from-to)161-70
Number of pages10
JournalMolecular and Cellular Probes
Volume21
Issue number3
DOIs
Publication statusPublished - 2007

Keywords / Materials (for Non-textual outputs)

  • DNA Probes
  • Drug Resistance, Bacterial
  • Genes, Bacterial
  • Microbial Sensitivity Tests
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Phenotype
  • Sensitivity and Specificity
  • Staining and Labeling
  • Staphylococcus aureus

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