DNA nanoswitch as a biosensor

Amy H. Buck, Colin J. Campbell, Paul Dickinson, Christopher P. Mountford, Helene C. Stoquert, Jonathan G. Terry, Stuart Evans, Lorraine M. Keane, Tsueu-Ju Su, Andrew R. Mount, Anthony J. Walton, John S. Beattie, Jason Crain, Peter Ghazal

Research output: Contribution to journalLetterpeer-review

Abstract

We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

Original languageEnglish
Pages (from-to)4724-4728
Number of pages5
JournalAnalytical Chemistry
Volume79
Issue number12
DOIs
Publication statusPublished - 15 Jun 2007

Keywords

  • SINGLE-NUCLEOTIDE POLYMORPHISMS
  • RESONANCE ENERGY-TRANSFER
  • HOLLIDAY JUNCTIONS
  • MOLECULAR BEACONS
  • 4-WAY
  • HYBRIDIZATION
  • DESIGN
  • PROBES
  • RNA

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