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Abstract / Description of output
We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.
Keywords / Materials (for Non-textual outputs)
- SINGLE-NUCLEOTIDE POLYMORPHISMS
- RESONANCE ENERGY-TRANSFER
- HOLLIDAY JUNCTIONS
- MOLECULAR BEACONS
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- 1 Finished
1/04/03 → 31/03/07