DNA nanoswitch as a biosensor

Amy H. Buck; Colin J. Campbell; Paul Dickinson; Christopher P. Mountford; Helene C. Stoquert; Jonathan G. Terry; Stuart Evans; Lorraine M. Keane; Tsueu-Ju Su; Andrew R. Mount; Anthony J. Walton; John S. Beattie; Jason Crain; Peter Ghazal

doi:10.1021/ac070251r

DNA nanoswitch as a biosensor

Amy H. Buck, Colin J. Campbell, Paul Dickinson, Christopher P. Mountford, Helene C. Stoquert, Jonathan G. Terry, Stuart Evans, Lorraine M. Keane, Tsueu-Ju Su, Andrew R. Mount, Anthony J. Walton, John S. Beattie, Jason Crain, Peter Ghazal

Research output: Contribution to journal › Letter › peer-review

Abstract / Description of output

We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

Original language	English
Pages (from-to)	4724-4728
Number of pages	5
Journal	Analytical Chemistry
Volume	79
Issue number	12
DOIs	https://doi.org/10.1021/ac070251r
Publication status	Published - 15 Jun 2007

Keywords / Materials (for Non-textual outputs)

SINGLE-NUCLEOTIDE POLYMORPHISMS
RESONANCE ENERGY-TRANSFER
HOLLIDAY JUNCTIONS
MOLECULAR BEACONS
4-WAY
HYBRIDIZATION
DESIGN
PROBES
RNA

Access to Document

10.1021/ac070251r

Beacon Project
Ghazal, P., Crain, J., Mount, A., Walton, A. & Terry, J.
UK central government bodies/local authorities, health and hospital authorities
1/04/03 → 31/03/07
Project: Research

Cite this

@article{c9083a7edfd14f6cae0b906da1671644,

title = "DNA nanoswitch as a biosensor",

abstract = "We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.",

keywords = "SINGLE-NUCLEOTIDE POLYMORPHISMS, RESONANCE ENERGY-TRANSFER, HOLLIDAY JUNCTIONS, MOLECULAR BEACONS, 4-WAY, HYBRIDIZATION, DESIGN, PROBES, RNA",

author = "Buck, {Amy H.} and Campbell, {Colin J.} and Paul Dickinson and Mountford, {Christopher P.} and Stoquert, {Helene C.} and Terry, {Jonathan G.} and Stuart Evans and Keane, {Lorraine M.} and Tsueu-Ju Su and Mount, {Andrew R.} and Walton, {Anthony J.} and Beattie, {John S.} and Jason Crain and Peter Ghazal",

year = "2007",

month = jun,

day = "15",

doi = "10.1021/ac070251r",

language = "English",

volume = "79",

pages = "4724--4728",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "12",

}

TY - JOUR

T1 - DNA nanoswitch as a biosensor

AU - Buck, Amy H.

AU - Campbell, Colin J.

AU - Dickinson, Paul

AU - Mountford, Christopher P.

AU - Stoquert, Helene C.

AU - Terry, Jonathan G.

AU - Evans, Stuart

AU - Keane, Lorraine M.

AU - Su, Tsueu-Ju

AU - Mount, Andrew R.

AU - Walton, Anthony J.

AU - Beattie, John S.

AU - Crain, Jason

AU - Ghazal, Peter

PY - 2007/6/15

Y1 - 2007/6/15

N2 - We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

AB - We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

KW - SINGLE-NUCLEOTIDE POLYMORPHISMS

KW - RESONANCE ENERGY-TRANSFER

KW - HOLLIDAY JUNCTIONS

KW - MOLECULAR BEACONS

KW - 4-WAY

KW - HYBRIDIZATION

KW - DESIGN

KW - PROBES

KW - RNA

U2 - 10.1021/ac070251r

DO - 10.1021/ac070251r

M3 - Letter

SN - 0003-2700

VL - 79

SP - 4724

EP - 4728

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 12

ER -

DNA nanoswitch as a biosensor

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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Projects

Beacon Project

Cite this