DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

D J McGeoch; A Dolan; M C Frame

doi:10.1093/nar/14.8.3435

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

D J McGeoch, A Dolan, M C Frame

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

We report the sequence of a 7800 base pair region of herpes simplex virus type 1 DNA, representing approximately 0.16 to 0.20 map units in the genome. This contains sequences transcribed into a leftward oriented set of five 3' coterminal mRNAs, together with two rightward transcribed flanking genes. One of the leftward genes encodes the virus's alkaline exonuclease, but the other gene products are uncharacterized. The amino acid sequence of one encoded protein suggested that it is a membrane embedded species. The DNA sequence is densely utilised, with two predicted out-of-frame overlaps of coding sequences, and probably six occurrences of promoter elements within coding sequences. Homologues of five of the genes were found for the distantly related Epstein-Barr virus, with a similar overall relative arrangement.

Original language	English
Pages (from-to)	3435-48
Number of pages	14
Journal	Nucleic Acids Research
Volume	14
Issue number	8
DOIs	https://doi.org/10.1093/nar/14.8.3435
Publication status	Published - 25 Apr 1986

Keywords / Materials (for Non-textual outputs)

Amino Acid Sequence
Base Composition
Base Sequence
Cloning, Molecular
DNA Restriction Enzymes
DNA, Viral
Exonucleases
Genes
Genes, Viral
Plasmids
Simplexvirus
Transcription, Genetic

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10.1093/nar/14.8.3435

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes
© I RL Press Limited 1986
Final published version, 1.23 MB

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title = "DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes",

abstract = "We report the sequence of a 7800 base pair region of herpes simplex virus type 1 DNA, representing approximately 0.16 to 0.20 map units in the genome. This contains sequences transcribed into a leftward oriented set of five 3' coterminal mRNAs, together with two rightward transcribed flanking genes. One of the leftward genes encodes the virus's alkaline exonuclease, but the other gene products are uncharacterized. The amino acid sequence of one encoded protein suggested that it is a membrane embedded species. The DNA sequence is densely utilised, with two predicted out-of-frame overlaps of coding sequences, and probably six occurrences of promoter elements within coding sequences. Homologues of five of the genes were found for the distantly related Epstein-Barr virus, with a similar overall relative arrangement.",

keywords = "Amino Acid Sequence, Base Composition, Base Sequence, Cloning, Molecular, DNA Restriction Enzymes, DNA, Viral, Exonucleases, Genes, Genes, Viral, Plasmids, Simplexvirus, Transcription, Genetic",

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T1 - DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

AU - McGeoch, D J

AU - Dolan, A

AU - Frame, M C

PY - 1986/4/25

Y1 - 1986/4/25

N2 - We report the sequence of a 7800 base pair region of herpes simplex virus type 1 DNA, representing approximately 0.16 to 0.20 map units in the genome. This contains sequences transcribed into a leftward oriented set of five 3' coterminal mRNAs, together with two rightward transcribed flanking genes. One of the leftward genes encodes the virus's alkaline exonuclease, but the other gene products are uncharacterized. The amino acid sequence of one encoded protein suggested that it is a membrane embedded species. The DNA sequence is densely utilised, with two predicted out-of-frame overlaps of coding sequences, and probably six occurrences of promoter elements within coding sequences. Homologues of five of the genes were found for the distantly related Epstein-Barr virus, with a similar overall relative arrangement.

AB - We report the sequence of a 7800 base pair region of herpes simplex virus type 1 DNA, representing approximately 0.16 to 0.20 map units in the genome. This contains sequences transcribed into a leftward oriented set of five 3' coterminal mRNAs, together with two rightward transcribed flanking genes. One of the leftward genes encodes the virus's alkaline exonuclease, but the other gene products are uncharacterized. The amino acid sequence of one encoded protein suggested that it is a membrane embedded species. The DNA sequence is densely utilised, with two predicted out-of-frame overlaps of coding sequences, and probably six occurrences of promoter elements within coding sequences. Homologues of five of the genes were found for the distantly related Epstein-Barr virus, with a similar overall relative arrangement.

KW - Amino Acid Sequence

KW - Base Composition

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA Restriction Enzymes

KW - DNA, Viral

KW - Exonucleases

KW - Genes

KW - Genes, Viral

KW - Plasmids

KW - Simplexvirus

KW - Transcription, Genetic

U2 - 10.1093/nar/14.8.3435

DO - 10.1093/nar/14.8.3435

M3 - Article

C2 - 3010237

SN - 0305-1048

VL - 14

SP - 3435

EP - 3448

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 8

ER -

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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