DNA vaccine delivery by densely-packed and short microprojection arrays to skin protects against vaginal HSV-2 challenge

Angela Shaulov Kask, Xianfeng Chen, Joshua O. Marshak, Lichun Dong, Misty Saracino, Dexiang Chen, Courtney Jarrahian, Mark A. Kendall, David M. Koelle*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

There is an unmet medical need for a prophylactic vaccine against herpes simplex virus (HSV). DNA vaccines and cutaneous vaccination have been tried for many applications, but few reports combine this vaccine composition and administration route. We compared DNA administration using the Nanopatch™, a solid microprojection device coated with vaccine comprised of thousands of short (110μm) densly-packed projections (70μm spacing), to standard intramuscular DNA vaccination in a mouse model of vaginal HSV-2 infection. A dose-response relationship was established for immunogenicity and survival in both vaccination routes. Appropriate doses administered by Nanopatch™ were highly immunogenic and enabled mouse survival. Vaginal HSV-2 DNA copy number day 1 post challenge correlated with survival, indicating that vaccine-elicited acquired immune responses can act quickly and locally. Solid, short, densely-packed arrays of microprojections applied to the skin are thus a promising route of administration for DNA vaccines.

Original languageEnglish
Pages (from-to)7483-7491
Number of pages9
JournalVaccine
Volume28
Issue number47
DOIs
Publication statusPublished - 3 Nov 2010

Keywords

  • DNA vaccine
  • Herpes simplex virus
  • Microprojection
  • Skin

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