Do polyamines contribute to plant cell wall assembly by forming amide bonds with pectins?

M Lenucci, G Piro, Janice G. Miller, G Dalessandro, S C Fry

Research output: Contribution to journalArticlepeer-review

Abstract

Two new reducing glycoconjugates [N-D-galacturonoyl-putrescinamide (GalA-Put) and NN'-di-D-galacturonoyl-putrescinamide (GalA-Put-GalA)] and homogalacturonan-putrescine (GalA(n)-Put) conjugates were synthesised as model compounds representing possible amide (isopeptide) linkage points between a polyamine and either one or two pectic galacturonate residues. The amide bond(s) were stable to cold acid and alkali (2 M TFA and 0.1 M NaOH at 25 degrees C) but rapidly hydrolysed by these agents at 100 degrees C. The amide bond(s) were resistant to Driselase and to all proteinases tested, although Driselase digested GalA(n)-Put, releasing fragments such as GalA(3)-Put-GalA(3). To trace the possible formation of GalA-polyamine amide bonds in vivo, we fed Arabidopsis and rose cell-cultures and chickpea internodes with [C-14]Put. About 20% of the C-14 taken up was released as (14) CO2, indicating some catabolism. An additional similar to 73% of the C-14 taken up (in Arabidopsis), or similar to 21% (in rose), became ethanol-insoluble, superficially suggestive of polysaccharide-Put covalent bonding. However, much of the ethanol-inextractable C-14 was subsequently extractable by acidified phenol or by cold I M TFA. The small proportion of radioactive material that stayed insoluble in both phenol and TFA was hydrolysable by Driselase or hot 6 M HCl, yielding C-14-oligopeptides and/or amino acids (including Asp, Glu, Gly, Ala and Val); no free C-14-polyamines were released by hot HCl. We conclude that if pectin-polyamine amide bonds are present, they are a very minor component of the cell walls of cultured rose and Arabidopsis cells and chickpea internodes. (c) 2005 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)2581-2594
Number of pages14
JournalPhytochemistry
Volume66
Issue number21
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Dive into the research topics of 'Do polyamines contribute to plant cell wall assembly by forming amide bonds with pectins?'. Together they form a unique fingerprint.

Cite this