Domains of DnaA protein involved in interaction with DnaB protein, and in unwinding the Escherichia coli chromosomal origin

J Marszalek, W Zhang, T R Hupp, C Margulies, K M Carr, S Cherry, J M Kaguni

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

DnaA protein of Escherichia coli is a sequence-specific DNA-binding protein required for the initiation of DNA replication from the chromosomal origin, oriC. It is also required for replication of several plasmids including pSC101, F, P-1, and R6K. A collection of monoclonal antibodies to DnaA protein has been produced and the primary epitopes recognized by them have been determined. These antibodies have also been examined for the ability to inhibit activities of DNA binding, ATP binding, unwinding of oriC, and replication of both an oriC plasmid, and an M13 single-stranded DNA with a proposed hairpin structure containing a DnaA protein-binding site. Replication of the latter DNA is dependent on DnaA protein by a mechanism termed ABC priming. These studies suggest regions of DnaA protein involved in interaction with DnaB protein, and in unwinding of oriC, or low-affinity binding of ATP.
Original languageEnglish
Pages (from-to)18535-42
Number of pages8
JournalJournal of Biological Chemistry
Volume271
Issue number31
Publication statusPublished - 2 Aug 1996

Keywords / Materials (for Non-textual outputs)

  • Adenosine Triphosphate
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Bacterial Proteins
  • Binding Sites
  • DNA Helicases
  • DNA Replication
  • DNA-Binding Proteins
  • DnaB Helicases
  • Epitopes
  • Escherichia coli
  • Molecular Sequence Data
  • Protein Conformation
  • Replication Origin

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