Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds

Yali Jia, Pierre O. Bagnaninchi, Ying Yang, Alicia El Haj, Monica T. Hinds, Sean J. Kirkpatrick, Ruikang K. Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Establishing a relationship between perfusion rate and fluid shear stress in a 3D cell culture environment is an ongoing and challenging task faced by tissue engineers. We explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low-and high-porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5 ml l min(-1). The DOCT results show that the behavior of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low-porosity and high-porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore, with a mean shear stress of 0.49 +/- 0.3 dyn.cm(-2) and 0.38 +/- 0.2 dyn.cm(-2), respectively. In addition, we show that the scaffold's porosity and interconnectivity can be quantified by combining analyses of the 3D structural and flow images obtained from DOCT. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3130345]

Original languageEnglish
Article number034014
Pages (from-to)-
Number of pages9
JournalJournal of Biomedical Optics
Volume14
Issue number3
DOIs
Publication statusPublished - 2009

Keywords

  • Doppler optical coherence tomography (DOCT)
  • local fluid flow
  • shear stress
  • interconnectivity
  • porous scaffold
  • tissue engineering
  • MINERALIZED MATRIX DEPOSITION
  • 3D PERFUSION CULTURE
  • BONE-CELLS
  • IN-VITRO
  • TISSUE
  • SENSITIVITY
  • INCREASES
  • CHONDROCYTES
  • ARCHITECTURE
  • ANGIOGRAPHY

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