Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response

Besaiz J. Sanchez-Sanchez, Jose M. Urbano, Kate Comber, Anca Dragu, Will Wood, Brian Stramer, Maria D. Martin-Bermudo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.

Original languageEnglish
Pages (from-to)1461-1470
Number of pages10
JournalCell Reports
Volume21
Issue number6
DOIs
Publication statusPublished - 7 Nov 2017

Keywords

  • BASEMENT-MEMBRANE PROTEINS
  • CELL-MIGRATION
  • MACROPHAGE-MIGRATION
  • EXTRACELLULAR-MATRIX
  • RAB PROTEINS
  • IV COLLAGEN
  • INTEGRIN
  • EXPRESSION
  • DEPOSITION
  • RECEPTOR

Fingerprint

Dive into the research topics of 'Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response'. Together they form a unique fingerprint.

Cite this