Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response

Besaiz J. Sanchez-Sanchez; Jose M. Urbano; Kate Comber; Anca Dragu; Will Wood; Brian Stramer; Maria D. Martin-Bermudo

doi:10.1016/j.celrep.2017.10.047

Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response

Besaiz J. Sanchez-Sanchez, Jose M. Urbano, Kate Comber, Anca Dragu, Will Wood, Brian Stramer, Maria D. Martin-Bermudo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.

Original language	English
Pages (from-to)	1461-1470
Number of pages	10
Journal	Cell Reports
Volume	21
Issue number	6
DOIs	https://doi.org/10.1016/j.celrep.2017.10.047
Publication status	Published - 7 Nov 2017

Keywords / Materials (for Non-textual outputs)

BASEMENT-MEMBRANE PROTEINS
CELL-MIGRATION
MACROPHAGE-MIGRATION
EXTRACELLULAR-MATRIX
RAB PROTEINS
IV COLLAGEN
INTEGRIN
EXPRESSION
DEPOSITION
RECEPTOR

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10.1016/j.celrep.2017.10.047

Wood W Drosophila embryonic hemocytes...
This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ ).
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@article{a8854a0cbc7d44d493df2f4b14c7305f,

title = "Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response",

abstract = "The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.",

keywords = "BASEMENT-MEMBRANE PROTEINS, CELL-MIGRATION, MACROPHAGE-MIGRATION, EXTRACELLULAR-MATRIX, RAB PROTEINS, IV COLLAGEN, INTEGRIN, EXPRESSION, DEPOSITION, RECEPTOR",

author = "Sanchez-Sanchez, {Besaiz J.} and Urbano, {Jose M.} and Kate Comber and Anca Dragu and Will Wood and Brian Stramer and Martin-Bermudo, {Maria D.}",

year = "2017",

month = nov,

day = "7",

doi = "10.1016/j.celrep.2017.10.047",

language = "English",

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AU - Sanchez-Sanchez, Besaiz J.

AU - Urbano, Jose M.

AU - Comber, Kate

AU - Dragu, Anca

AU - Wood, Will

AU - Stramer, Brian

AU - Martin-Bermudo, Maria D.

PY - 2017/11/7

Y1 - 2017/11/7

N2 - The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.

AB - The most prominent developmental function attributed to the extracellular matrix (ECM) is cell migration. While cells in culture can produce ECM to migrate, the role of ECM in regulating developmental cell migration is classically viewed as an exogenous matrix presented to the moving cells. In contrast to this view, we show here that Drosophila embryonic hemocytes deposit their own laminins in streak-like structures to migrate efficiently throughout the embryo. With the help of transplantation experiments, live microscopy, and image quantification, we demonstrate that autocrine-produced laminin regulates hemocyte migration by controlling lamellipodia dynamics, stability, and persistence. Proper laminin deposition is regulated by the RabGTPase Rab8, which is highly expressed and required in hemocytes for lamellipodia dynamics and migration. Our results thus support a model in which, during embryogenesis, the Rab8-regulated autocrine deposition of laminin reinforces directional and effective migration by stabilizing cellular protrusions and strengthening otherwise transient adhesion states.

KW - BASEMENT-MEMBRANE PROTEINS

KW - CELL-MIGRATION

KW - MACROPHAGE-MIGRATION

KW - EXTRACELLULAR-MATRIX

KW - RAB PROTEINS

KW - IV COLLAGEN

KW - INTEGRIN

KW - EXPRESSION

KW - DEPOSITION

KW - RECEPTOR

U2 - 10.1016/j.celrep.2017.10.047

DO - 10.1016/j.celrep.2017.10.047

M3 - Article

SN - 2211-1247

VL - 21

SP - 1461

EP - 1470

JO - Cell Reports

JF - Cell Reports

IS - 6

ER -

Drosophila Embryonic Hemocytes Produce Laminins to Strengthen Migratory Response

Abstract

Keywords / Materials (for Non-textual outputs)

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