Dual roles of Incenp crucial to the assembly of the acentrosomal metaphase spindle in female meiosis

Nathalie Colombie, Fiona Cullen, Amy Brittle, Janet Jang, Bill Earnshaw, Maria Carmena, Kim McKim, Hiroyuki Ohkura

Research output: Contribution to journalArticlepeer-review

Abstract

Spindle formation in female meiosis differs from mitosis in many animals, as it takes place independently from centrosomes, and the molecular requirements of this pathway remain to be understood. Here we report two crucial roles of Incenp, an essential subunit of the chromosomal passenger complex (the Aurora B complex), in centrosome-independent spindle formation in Drosophila female meiosis. Firstly, the initial assembly of spindle microtubules is drastically delayed in an incenp mutant. This clearly demonstrates, for the first time, a crucial role for Incenp in chromosome-driven spindle microtubule assembly in living oocytes. Additionally, Incenp is necessary to stabilise the equatorial region of the metaphase I spindle, in contrast to mitosis, where the equivalent function becomes prominent after anaphase onset. Our analysis suggests that Subito, a kinesin-6 protein, cooperates with Incenp for this latter function, but not microtubule assembly. We propose that the two functions of Incenp are part of the mechanisms that compensate for the lack of centrosomes during meiotic spindle formation.
Original languageEnglish
Pages (from-to)3239-3246
JournalDevelopment
Volume135
Issue number19
Early online date28 Aug 2008
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Drosophila
  • Aurora
  • Kinase
  • Microtubule
  • Meiosis

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