Dual targeting of EGFR and ERBB2 pathways produces a synergistic effect on cancer cell proliferation and migration in vitro

Mark Gray, Seungmee Lee, Ashleigh L. McDowell, Matthew Erskine, Q.T.M. Loh, Olivia Grice, David Argyle, Guraa Bergkvist

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the epidermal growth factor receptor (EGFR/ERBB) gene family are frequently dysregulated in a range of human cancers, and therapeutics targeting these proteins are in clinical use. We hypothesised that similar pathways are involved in feline and canine tumours and that the same drugs may be of clinical use in veterinary patients. We investigated EGFR and ERBB2 targeting using a panel of feline and canine cell lines. EGFR and ERBB2 was targeted with siRNAs or TKIs and their effect on cellular proliferation, colony formation and migration was investigated in vitro. Here we report that EGFR and ERBB2 combined siRNA targeting produced synergistic effects in feline and canine cell lines similar to that reported in human cell lines. We conclude that dual EGFR and ERBB2 targeting using TKIs should be further evaluated as a potential new therapeutic strategy in feline head and neck and mammary tumours and canine mammary tumours.
Original languageEnglish
Pages (from-to)890-909
JournalVeterinary and Comparative Oncology
Volume15
Issue number3
Early online date27 May 2016
DOIs
Publication statusPublished - 8 Aug 2017

Keywords

  • Cell signaling
  • Comparative oncology
  • In vitro models
  • Gene Therapy
  • small animal
  • tumour biology
  • Tyrosine kinase

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