Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies

R. Dundas, S Boyle, CO Bellamy, W Hawkins, OJ Garden, JA Ross, W Bickmore

Research output: Contribution to journalArticlepeer-review

Abstract

Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.

Original languageEnglish
Pages (from-to)1321-1322
Number of pages2
JournalJournal of Histochemistry and Cytochemistry
Volume49
Issue number10
Publication statusPublished - Oct 2001

Keywords

  • fluorescence in situ hybridization
  • chromosome painting
  • Y-chromosome chimerism
  • liver allografts

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