Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.
|Number of pages||2|
|Journal||Journal of Histochemistry and Cytochemistry|
|Publication status||Published - Oct 2001|
- fluorescence in situ hybridization
- chromosome painting
- Y-chromosome chimerism
- liver allografts