Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies

R. Dundas; S Boyle; CO Bellamy; W Hawkins; OJ Garden; JA Ross; W Bickmore

Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies

R. Dundas, S Boyle, CO Bellamy, W Hawkins, OJ Garden, JA Ross, W Bickmore

Research output: Contribution to journal › Article › peer-review

Abstract

Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.

Original language	English
Pages (from-to)	1321-1322
Number of pages	2
Journal	Journal of Histochemistry & Cytochemistry (JHC)
Volume	49
Issue number	10
Publication status	Published - Oct 2001

Keywords / Materials (for Non-textual outputs)

fluorescence in situ hybridization
chromosome painting
Y-chromosome chimerism
liver allografts

Cite this

@article{15a6c9997a7a4ce5aae91a6aa644efaf,

title = "Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies",

abstract = "Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.",

keywords = "fluorescence in situ hybridization, chromosome painting, Y-chromosome chimerism, liver allografts",

author = "R. Dundas and S Boyle and CO Bellamy and W Hawkins and OJ Garden and JA Ross and W Bickmore",

year = "2001",

month = oct,

language = "English",

volume = "49",

pages = "1321--1322",

journal = "Journal of Histochemistry \& Cytochemistry (JHC)",

issn = "0022-1554",

publisher = "Histochemical Society Inc.",

number = "10",

}

TY - JOUR

T1 - Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies

AU - Dundas, R.

AU - Boyle, S

AU - Bellamy, CO

AU - Hawkins, W

AU - Garden, OJ

AU - Ross, JA

AU - Bickmore, W

PY - 2001/10

Y1 - 2001/10

N2 - Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.

AB - Combining fluorescence in situ hybridization (FISH) and indirect immunofluorescence staining of protein markers provides a highly specific method for identifying chromosomes in phenotypically defined cells and tissues. We developed a technique enabling dual chromosome painting and immunofluorescence staining of archival formalin-fixed, paraffin-embedded material, and used this to phenotype chimeric cells in female-to-male human liver transplants.

KW - fluorescence in situ hybridization

KW - chromosome painting

KW - Y-chromosome chimerism

KW - liver allografts

M3 - Article

SN - 0022-1554

VL - 49

SP - 1321

EP - 1322

JO - Journal of Histochemistry & Cytochemistry (JHC)

JF - Journal of Histochemistry & Cytochemistry (JHC)

IS - 10

ER -

Dual Y-chromosome painting and immunofluorescence staining of archival human liver transplant biopsies

Abstract

Keywords / Materials (for Non-textual outputs)

Fingerprint

Cite this