Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging

Assel Baibek; Muhammed Üçüncü; Bryn Short; Gordon Ramage; Annamaria Lilienkampf; Mark Bradley

doi:10.1039/d0cc08177a

Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging

Assel Baibek, Muhammed Üçüncü, Bryn Short, Gordon Ramage, Annamaria Lilienkampf, Mark Bradley

Research output: Contribution to journal › Article › peer-review

Abstract

The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.

Original language	English
Pages (from-to)	1899-1902
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	15
Early online date	19 Jan 2021
DOIs	https://doi.org/10.1039/d0cc08177a
Publication status	Published - 23 Feb 2021

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10.1039/d0cc08177a

d0cc08177aFinal published version, 4.34 MB

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond
Bradley, M. (Principal Investigator), Bradley, M. (Principal Investigator), Dhaliwal, K. (Co-investigator), Dhaliwal, K. (Co-investigator), Haslett, C. (Co-investigator), Henderson, R. (Co-investigator), Walsh, T. (Co-investigator) & Walsh, T. (Co-investigator)
Engineering and Physical Sciences Research Council
1/01/19 → 1/06/23
Project: Research
Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging
Bradley, M. (Principal Investigator)
EPSRC
1/10/13 → 31/03/19
Project: Research

Cite this

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abstract = "The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.",

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AU - Üçüncü, Muhammed

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AU - Ramage, Gordon

AU - Lilienkampf, Annamaria

AU - Bradley, Mark

PY - 2021/2/23

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AB - The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, allowing multiplexed imaging and identification of microbial species in a co-culture of fungi and Gram-positive and Gram-negative bacteria.

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Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging

Abstract

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Projects

EPSRC IRC Proteus - Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging - Lifetime and Beyond

Multiplexed 'Touch and Tell' Optical Molecular Sensing and Imaging

Cite this