Projects per year
Abstract / Description of output
Cohesin organizes the genome by forming intra-chromosomal loops and inter-sister chromatid linkages. During gamete formation by meiosis, chromosomes are reshaped to support crossover recombination and two consecutive rounds of chromosome segregation. Here we show that meiotic chromosomes are organised into functional domains by Eco1 acetyltransferase-dependent positioning of both chromatin loops and sister chromatid cohesion in budding yeast. Eco1 acetylates the Smc3 cohesin subunit in meiotic S phase to establish chromatin boundaries, independently of DNA replication. Boundary formation by Eco1 is critical for prophase exit and for the maintenance of cohesion until meiosis II, but is independent of the ability of Eco1 to antagonize the cohesin-release factor, Wpl1. Conversely, prevention of cohesin release by Wpl1 is essential for centromeric cohesion, kinetochore monoorientation and co-segregation of sister chromatids in meiosis I. Our findings establish Eco1 as a key determinant of chromatin boundaries and cohesion positioning, revealing how local chromosome structuring directs genome transmission into gametes.
Original language | English |
---|---|
Article number | e74447 |
Number of pages | 39 |
Journal | eLIFE |
Volume | 11 |
DOIs | |
Publication status | Published - 1 Feb 2022 |
Keywords / Materials (for Non-textual outputs)
- cohesin
- meiosis
- Eco1
- Wpl1
- Hi-C
- chromatin loop
- loop extrusion
- boundary
- pericentromere border
- Saccharomyces cerevisiae
Fingerprint
Dive into the research topics of 'Eco1-dependent cohesin acetylation anchors chromatin loops and cohesion to define functional meiotic chromosome domains'. Together they form a unique fingerprint.-
-
-
Synthetic chromosomes to decipher requirements for optimal transmission of DNA in yeast
16/09/19 → 15/03/23
Project: Research