Editing independent effects of ADARs on the miRNA/siRNA pathways

Bret S E Heale; Liam P Keegan; Leeanne McGurk; Gracjan Michlewski; James Brindle; Chloe M Stanton; Javier F Caceres; Mary A O'Connell

doi:10.1038/emboj.2009.244

Editing independent effects of ADARs on the miRNA/siRNA pathways

Bret S E Heale, Liam P Keegan, Leeanne McGurk, Gracjan Michlewski, James Brindle, Chloe M Stanton, Javier F Caceres, Mary A O'Connell

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Adenosine deaminases acting on RNA (ADARs) are best known for altering the coding sequences of mRNA through RNA editing, as in the GluR-B Q/R site. ADARs have also been shown to affect RNA interference (RNAi) and microRNA processing by deamination of specific adenosines to inosine. Here, we show that ADAR proteins can affect RNA processing independently of their enzymatic activity. We show that ADAR2 can modulate the processing of mir-376a2 independently of catalytic RNA editing activity. In addition, in a Drosophila assay for RNAi deaminase-inactive ADAR1 inhibits RNAi through the siRNA pathway. These results imply that ADAR1 and ADAR2 have biological functions as RNA-binding proteins that extend beyond editing per se and that even genomically encoded ADARs that are catalytically inactive may have such functions.

Original language	English
Pages (from-to)	3145-3156
Number of pages	12
Journal	EMBO Journal
Volume	28
Issue number	20
DOIs	https://doi.org/10.1038/emboj.2009.244
Publication status	Published - Oct 2009

Keywords / Materials (for Non-textual outputs)

Drosophila
DSH1
microprocessor
RNA editing
RNA interference

Access to Document

10.1038/emboj.2009.244

Editing independent effects of ADARs on the miRNA siRNA pathways
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, andreproductioninanymedium, providedthe original authorandsource are credited.This licensedoesnot permit commercial exploitation or the creation of derivativeworks without specific permission.
Final published version, 1.93 MB

http://www.nature.com/emboj/journal/v28/n20/full/emboj2009244a.html

Cite this

@article{9bff5efb2c5e442d877d565595dae92e,

title = "Editing independent effects of ADARs on the miRNA/siRNA pathways",

abstract = "Adenosine deaminases acting on RNA (ADARs) are best known for altering the coding sequences of mRNA through RNA editing, as in the GluR-B Q/R site. ADARs have also been shown to affect RNA interference (RNAi) and microRNA processing by deamination of specific adenosines to inosine. Here, we show that ADAR proteins can affect RNA processing independently of their enzymatic activity. We show that ADAR2 can modulate the processing of mir-376a2 independently of catalytic RNA editing activity. In addition, in a Drosophila assay for RNAi deaminase-inactive ADAR1 inhibits RNAi through the siRNA pathway. These results imply that ADAR1 and ADAR2 have biological functions as RNA-binding proteins that extend beyond editing per se and that even genomically encoded ADARs that are catalytically inactive may have such functions.",

keywords = "Drosophila, DSH1, microprocessor, RNA editing, RNA interference",

author = "Heale, {Bret S E} and Keegan, {Liam P} and Leeanne McGurk and Gracjan Michlewski and James Brindle and Stanton, {Chloe M} and Caceres, {Javier F} and O'Connell, {Mary A}",

year = "2009",

month = oct,

doi = "10.1038/emboj.2009.244",

language = "English",

volume = "28",

pages = "3145--3156",

journal = "EMBO Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell",

number = "20",

}

TY - JOUR

T1 - Editing independent effects of ADARs on the miRNA/siRNA pathways

AU - Heale, Bret S E

AU - Keegan, Liam P

AU - McGurk, Leeanne

AU - Michlewski, Gracjan

AU - Brindle, James

AU - Stanton, Chloe M

AU - Caceres, Javier F

AU - O'Connell, Mary A

PY - 2009/10

Y1 - 2009/10

N2 - Adenosine deaminases acting on RNA (ADARs) are best known for altering the coding sequences of mRNA through RNA editing, as in the GluR-B Q/R site. ADARs have also been shown to affect RNA interference (RNAi) and microRNA processing by deamination of specific adenosines to inosine. Here, we show that ADAR proteins can affect RNA processing independently of their enzymatic activity. We show that ADAR2 can modulate the processing of mir-376a2 independently of catalytic RNA editing activity. In addition, in a Drosophila assay for RNAi deaminase-inactive ADAR1 inhibits RNAi through the siRNA pathway. These results imply that ADAR1 and ADAR2 have biological functions as RNA-binding proteins that extend beyond editing per se and that even genomically encoded ADARs that are catalytically inactive may have such functions.

AB - Adenosine deaminases acting on RNA (ADARs) are best known for altering the coding sequences of mRNA through RNA editing, as in the GluR-B Q/R site. ADARs have also been shown to affect RNA interference (RNAi) and microRNA processing by deamination of specific adenosines to inosine. Here, we show that ADAR proteins can affect RNA processing independently of their enzymatic activity. We show that ADAR2 can modulate the processing of mir-376a2 independently of catalytic RNA editing activity. In addition, in a Drosophila assay for RNAi deaminase-inactive ADAR1 inhibits RNAi through the siRNA pathway. These results imply that ADAR1 and ADAR2 have biological functions as RNA-binding proteins that extend beyond editing per se and that even genomically encoded ADARs that are catalytically inactive may have such functions.

KW - Drosophila

KW - DSH1

KW - microprocessor

KW - RNA editing

KW - RNA interference

UR - http://www.scopus.com/inward/record.url?scp=70350336432&partnerID=8YFLogxK

U2 - 10.1038/emboj.2009.244

DO - 10.1038/emboj.2009.244

M3 - Article

C2 - 19713932

SN - 0261-4189

VL - 28

SP - 3145

EP - 3156

JO - EMBO Journal

JF - EMBO Journal

IS - 20

ER -

Editing independent effects of ADARs on the miRNA/siRNA pathways

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Cite this