Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

Lourdes Díaz-Rodríguez; Olga García-Martínez; Manuel Arroyo-Morales; Belén Rubio-Ruiz; Concepción Ruiz

doi:10.1038/aps.2010.129

Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

Lourdes Díaz-Rodríguez, Olga García-Martínez, Manuel Arroyo-Morales, Belén Rubio-Ruiz, Concepción Ruiz

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.

METHODS: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.

RESULTS: Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).

CONCLUSION: These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.

Original language	English
Pages (from-to)	1495-9
Number of pages	5
Journal	Acta Pharmacologica Sinica
Volume	31
Issue number	11
DOIs	https://doi.org/10.1038/aps.2010.129
Publication status	Published - Nov 2010

Keywords

Acetaminophen
Anti-Inflammatory Agents, Non-Steroidal
Antigen Presentation
Antigens, CD80
Antigens, CD86
Cell Culture Techniques
Cell Line, Tumor
Cell Proliferation
Dose-Response Relationship, Drug
Flow Cytometry
HLA-DR Antigens
Humans
Osteoblasts
Osteocalcin
Phagocytosis
T-Lymphocytes

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10.1038/aps.2010.129

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title = "Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63",

abstract = "AIM: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.METHODS: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.RESULTS: Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).CONCLUSION: These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.",

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author = "Lourdes D{\'i}az-Rodr{\'i}guez and Olga Garc{\'i}a-Mart{\'i}nez and Manuel Arroyo-Morales and Bel{\'e}n Rubio-Ruiz and Concepci{\'o}n Ruiz",

year = "2010",

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doi = "10.1038/aps.2010.129",

language = "English",

volume = "31",

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T1 - Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

AU - Díaz-Rodríguez, Lourdes

AU - García-Martínez, Olga

AU - Arroyo-Morales, Manuel

AU - Rubio-Ruiz, Belén

AU - Ruiz, Concepción

PY - 2010/11

Y1 - 2010/11

N2 - AIM: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.METHODS: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.RESULTS: Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).CONCLUSION: These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.

AB - AIM: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.METHODS: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.RESULTS: Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).CONCLUSION: These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.

KW - Acetaminophen

KW - Anti-Inflammatory Agents, Non-Steroidal

KW - Antigen Presentation

KW - Antigens, CD80

KW - Antigens, CD86

KW - Cell Culture Techniques

KW - Cell Line, Tumor

KW - Cell Proliferation

KW - Dose-Response Relationship, Drug

KW - Flow Cytometry

KW - HLA-DR Antigens

KW - Humans

KW - Osteoblasts

KW - Osteocalcin

KW - Phagocytosis

KW - T-Lymphocytes

U2 - 10.1038/aps.2010.129

DO - 10.1038/aps.2010.129

M3 - Article

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VL - 31

SP - 1495

EP - 1499

JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

SN - 1671-4083

IS - 11

ER -

Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

Abstract

Keywords

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