Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

Lourdes Díaz-Rodríguez, Olga García-Martínez, Manuel Arroyo-Morales, Belén Rubio-Ruiz, Concepción Ruiz

Research output: Contribution to journalArticlepeer-review

Abstract

AIM: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63.

METHODS: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker.

RESULTS: Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR).

CONCLUSION: These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.

Original languageEnglish
Pages (from-to)1495-9
Number of pages5
JournalActa Pharmacologica Sinica
Volume31
Issue number11
DOIs
Publication statusPublished - Nov 2010

Keywords

  • Acetaminophen
  • Anti-Inflammatory Agents, Non-Steroidal
  • Antigen Presentation
  • Antigens, CD80
  • Antigens, CD86
  • Cell Culture Techniques
  • Cell Line, Tumor
  • Cell Proliferation
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • HLA-DR Antigens
  • Humans
  • Osteoblasts
  • Osteocalcin
  • Phagocytosis
  • T-Lymphocytes

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