Effect of base sequence on g-wire formation in solution

Lea Spindler*, Martin Rigler, Irena Drevenšek-Olenik, Nason Maani, Mateus Webba da Silva

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating.
Original languageEnglish
JournalJournal of Nucleic Acids
Volume2010
DOIs
Publication statusPublished - 3 Jun 2010

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