Effect of protease-activated receptor-2 deficiency on allergic dermatitis in the mouse ear

Junichi Kawagoe, Toshiaki Takizawa, Jiro Matsumoto, Masaki Tamiya, Stephen Meek, Andrew J H Smith, Gary Hunter, Robin Plevin, Naohiro Saito, Toru Kanke, Mikio Fujii, Yasushi Wada

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

To investigate the involvement of protease-activated receptor-2 (PAR-2) in allergic dermatitis, we generated PAR-2-deficient (PAR-2(-/-)) mice. Ear thickness, contact hypersensitivity (CH) induced by topical application of picryl chloride (PC) or oxazolone (Ox) after sensitization, and vascular permeability after ear passive cutaneous anaphylaxis (PCA) were compared between wild-type (WT) and PAR-2(-/-) mice. Ear thickness was almost the same in untreated WT and PAR-2(-/-) mice. Topical application of PC or Ox thickened the ears at 6, 24 and 48 h after challenge with a peak at 24 h in WT mice. In PAR-2(-/-) mice, the ear swelling induced by both PC and Ox was suppressed at every time point, and significant inhibition was found at 24 h in PC-induced CH and at 24 and 48 h in Ox-induced CH. Histopathological observation of the ears at 24 h after challenge revealed that PC- or Ox-induced ear edema and infiltration of inflammatory cells in WT mice were greatly attenuated in PAR-2(-/-) mice. The vascular permeability in the ears after PCA was not different between WT and PAR-2(-/-) mice. These results strongly suggest that PAR-2 plays a crucial role in type IV allergic dermatitis but not in type I allergic dermatitis.
Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalJapanese Journal of Pharmacology
Issue number1
Publication statusPublished - Jan 2002

Keywords / Materials (for Non-textual outputs)

  • Adjuvants, Immunologic/adverse effects
  • Animals
  • Dermatitis, Allergic Contact/genetics
  • Dermatitis, Allergic Contact/immunology
  • Dermatitis, Allergic Contact/pathology
  • Disease Models, Animal
  • Drug Hypersensitivity/genetics
  • Drug Hypersensitivity/immunology
  • Drug Hypersensitivity/pathology
  • Ear/pathology
  • Gene Deletion
  • Male
  • Mice
  • Mice, Knockout
  • Microscopy
  • Oxazolone/adverse effects
  • Picryl Chloride/adverse effects
  • Receptor, PAR-2
  • Receptors, Thrombin/deficiency
  • Receptors, Thrombin/genetics
  • Staining and Labeling
  • Time Factors


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