@article{3c64841bfdc342ac9bd2f4eb0c22df55,
title = "Effect of serum sample storage temperature on metabolomic and proteomic biomarkers",
abstract = "Prospective biomarker studies can be used to identify biomarkers predictive of disease onset. However, if serum biomarkers are measured years after their collection, the storage conditions might affect analyte concentrations. Few data exists concerning which metabolites and proteins are affected by storage at - 20 °C vs - 80 °C. Our objectives were to document analytes affected by storage of serum samples at - 20 °C vs - 80 °C, and to identify those indicative of the storage temperature. We utilized liquid chromatography tandem mass spectrometry and Luminex to quantify 300 analytes from serum samples of 16 Finnish individuals with type 1 diabetes, with split-aliquot samples stored at - 80 °C and - 20 °C for a median of 4.2 years. Results were validated in 315 Finnish and 916 Scottish individuals with type 1 diabetes, stored at - 20 °C and at - 80 °C, respectively. After quality control, we analysed 193 metabolites and proteins of which 120 were apparently unaffected and 15 clearly susceptible to storage at - 20 °C vs - 80 °C. Further, we identified serum glutamate/glutamine ratio greater than 0.20 as a biomarker of storage at - 20 °C vs - 80 °C. The results provide a catalogue of analytes unaffected and affected by storage at - 20 °C vs - 80 °C and biomarkers indicative of sub-optimal storage.",
author = "Erkka Valo and Marco Colombo and Niina Sandholm and McGurnaghan, {Stuart J} and Blackbourn, {Luke A K} and Dunger, {David B} and McKeigue, {Paul M} and Carol Forsblom and Per-Henrik Groop and Colhoun, {Helen M} and Charles Turner and Dalton, {R Neil}",
note = "Funding Information: This study was supported by funding from Juvenile Diabetes Research Foundation (Ref. 1-SRA-2016-333-M-R); Chief Scientist Office (Ref. ETM/47); Diabetes UK (Ref. 10/0004010); Folkh{\"a}lsan Research Foundation; the Wilhelm and Else Stockmann Foundation; the Liv och H{\"a}lsa Society; the Novo Nordisk Foundation (NNF OC0013659); the Helsinki University Hospital Research Funds; the Academy of Finland (299200 and 316664); the Finnish Diabetes Research Foundation; and the University of Helsinki Research Foundation. In-kind contribution from Scottish Diabetes Research Network. Funding Information: The skilled technical assistance of Maikki Parkkonen, Mira Korolainen, Anna-Reetta Salonen, Anna Sandelin, and Jaana Tuomikangas is gratefully acknowledged. The authors also acknowledge all the physicians and nurses at each center participating in the collection of patients. Finally, we thank all the FinnDiane Study Group investigators (Supplementary Table 5). We thank the SDRN Type 1 Bioresource investigators: John Chalmers, Andrew Collier, Colin Fischbacher, Fiona Green, Robert Lindsay, John McKnight, Sandra MacRury, Colin Palmer, Alan Patrick, Donald Pearson, John Petrie and Sandeep Thekkepat. We thank the nurses across the participating sites in SDRN Type 1 Bioresource, and those with diabetes who took part. Funding Information: P-HG has received investigator research grants from Eli Lilly and Roche, lecture honoraria from Astellas, AstraZeneca, Boehringer Ingelheim, Eli Lilly, Elo Water, Genzyme, MSD, Novartis, Novo Nordisk, and Sanofi. P-HG is an advisor for AbbVie, Astellas, AstraZeneca, Bayer, Boehringer Ingelheim, Cebix, Eli Lilly, Janssen, MSD, Novartis, Novo Nordisk and Sanofi. HMC receives research support and honorarium and is also a member of the advisory panels and speaker{\textquoteright}s bureaus for Sanofi Aventis, Regeneron, and Eli Lilly. HMC has been a member of DSMB of the Advisory Panel for the CANTOS Trial (Novartis Pharmaceuticals). HMC also receives or has recently received a non-binding research support from Pfizer Inc., and AstraZeneca LP. HMC is a shareholder of Roche Pharmaceuticals and Bayer. RND and CT are Directors of SpOtOn Clinical Diagnostics Ltd. EV, MC, NS, SJM, LAKB, DBD, PMM, and CF declare that there is no duality of interest associated with their contribution to this manuscript. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = mar,
day = "17",
doi = "10.1038/s41598-022-08429-0",
language = "English",
volume = "12",
pages = "4571",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}