Effects of cellular mediator agonists on cortisol and steroid acute regulatory (StAR) protein in bovine zona fasciculata (ZF) cells

S D Morley, J L Hobkirk, R J Hall, M Nicol, J I Mason, B C Williams

Research output: Contribution to journalArticlepeer-review

Abstract

Primary cultures of bovine ZF cells were incubated for 1 h or 6h with the agonists 8-bromo-cyclic AMP (8-Br-cAMP) an activator of protein kinase A (PKA), the phorbol ester phorbol 12-myristate 13-acetate (PMA) a protein kinase C (PKC) activator, the Ca2+ ionophore, A23187, or the L-type Ca2+ channel agonist Bay K8644. Both cortisol secretion (determined by radioimmunoassay of cell medium) and cellular StAR protein levels (quantified by western blotting) were significantly increased at 6h, by all agonists. However, while all agonists stimulated cortisol secretion at 1h, StAR protein levels remained unchanged by these treatments. We conclude that in bovine ZF cells, StAR protein synthesis can be regulated by mechanisms involving activation of PKA, PKC and Ca2+ influx. However, a net increase in cellular StAR protein does not appear to be essential for the initiation for the first stage of acute steroidogenesis.
Original languageEnglish
Pages (from-to)603-8
Number of pages6
JournalEndocrine research
Volume26
Issue number4
Publication statusPublished - Nov 2000

Keywords

  • 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Animals
  • Calcimycin
  • Calcium Channel Agonists
  • Cattle
  • Cells, Cultured
  • Cyclic AMP-Dependent Protein Kinases
  • Enzyme Activation
  • Hydrocortisone
  • Ionophores
  • Phosphoproteins
  • Tetradecanoylphorbol Acetate
  • Zona Fasciculata

Fingerprint

Dive into the research topics of 'Effects of cellular mediator agonists on cortisol and steroid acute regulatory (StAR) protein in bovine zona fasciculata (ZF) cells'. Together they form a unique fingerprint.

Cite this