Effects of vagotomy on systemic and regional inflammation in ovine fetus near term

L. Garzoni, Liu, H.L., L.d. Durosier, M. Cao, P. Burns, G. Fecteau, A. Desrochers, N. Patey, C. Faure, M.g. Frasch

Research output: Contribution to journalMeeting abstractpeer-review

Abstract

Introduction: Higher levels of vagal activity are associated with lower systemic levels of pro-inflammatory cytokines and activation of macrophages, mediated via cholinergic anti-inflammatory pathway (CAP) by the vagus nerve. The behaviour of this neuroimmunological network in fetus is unknown. We hypothesized that bilateral cervical vagotomy (Vx) will potentiate the lipopolysaccharide (LPS)-induced inflammation with higher systemic inflammatory cytokines and ileum macrophage activation. Methods: Near-term fetal sheep were prepared with vascular catheters (N = 18 controls (NaCl i.v.), N = 16 LPS i.v., N = 5 Vx + LPS i.v.). Arterial blood samples were drawn at baseline and seven selected time points to profile inflammation. At 54 h post LPS, necropsy was performed. The plasma levels of IL-6 and TNF-α (ELISA) and the Iba1 + cell intensity in terminal ileum were used to quantify the degree of inflammation and macrophage activation. Results are reported for P < 0.05 adjusting for multiple comparisons as median (25-75 quartile ranges). Results: In the LPS group, but not in the control and Vx + LPS groups, at 3 h post LPS, IL-6, but not TNF-α, was elevated compared to baseline: 0 (0;0) pg/ml; 293 (33;462) pg/ml; 53 (0;267) pg\ml; 0 (0;0) pg/ml (Controls; LPS; Vx + LPS; mean baseline across all groups, respectively). IL-6 values were higher at 3 h in the LPS group vs. control, but not vs. Vx + LPS group; Vx + LPS did not differ from controls. In line with Vx effect on systemic inflammation, Iba1 + cell intensity was lower in Vx + LPS vs. LPS group but not different from controls. Discussion: In contrast to our hypothesis, we found that abolition of vagus-mediated CAP activity results in a decrease of systemic inflammation and a concomitant decrease of the regional ileum inflammation returning it to the baseline levels. A possible reason for this seeming discrepancy with findings in postnatal developing and adult brain–innate immune system interface may be the degree of its maturation.
Original languageEnglish
Pages (from-to)104
Number of pages1
JournalInternational journal of developmental neuroscience
Volume47
DOIs
Publication statusPublished - 1 Dec 2015
Externally publishedYes

Fingerprint Dive into the research topics of 'Effects of vagotomy on systemic and regional inflammation in ovine fetus near term'. Together they form a unique fingerprint.

Cite this